The isolates were cultivated aerobically on Trypticase Soy Agar supplemented with 5% bovine blood (bovine blood agar) (Tammer BioLab Oy, Tampere, Finland) at 37°C for 24 h, and the pureness of the cultures was controlled. One colony from a bovine blood agar culture of each isolate was transferred into tubes with 5 mL Müller-Hinton broth and incubated overnight at 37°C. DNA for whole-genome sequencing was extracted, using an Easy-DNATM Kit for genomic DNA isolation (Invitrogen Life Technologies, Carlsbad, CA, United States; now Applied Biosystems-Thermo Fisher Scientific Corp., Waltham, MA, United States).
Easy dnatm kit for genomic dna isolation
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Easy-DNA™ Kit is a laboratory product designed for the isolation of genomic DNA. It provides a simple and efficient method for extracting DNA from various biological samples.
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Lab products found in correlation
2 protocols using easy dnatm kit for genomic dna isolation
1
Bacterial Isolate Cultivation and DNA Extraction
2
Antibiotic Resistance Profiling of Shigella sonnei
Check if the same lab product or an alternative is used in the 5 most similar protocols
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12 Shigella sonnei strains were isolated from different patients with either diarrhea or dysentery in four cities in Jiangsu province, China. Resistance profile to 9 antibiotics (AMC, CFT, CTX, GEN, NAL, NOR, TBT and SMZ) as previously described for each strain was provided by Jiangsu Provincial CDC based on routine screening. Isolates of Shigella flexneri were plated on trypticase soya agar (TSA). Picked-up single colony was then inoculated in 5ml trypticase soya broth (TSB) and incubated overnight at 37°C with shaking rate of 200 rpm. DNA isolation was performed using Easy-DNA TM Kit for genomic DNA isolation (Invitrogen Life Technologies, Carlsbad, CA, USA)
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