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Fvb and 129 e mice

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FVB and 129-E mice are inbred laboratory mouse strains commonly used in research. FVB mice are characterized by their high reproductive performance and large pronuclei, making them suitable for microinjection studies. The 129-E strain is known for its embryonic stem cell derivation and is often used in gene targeting experiments. Both strains provide reliable and standardized model organisms for various scientific investigations.

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Lab products found in correlation

Clone rmp1 14, by BioXCell (1 mentions)

2 protocols using fvb and 129 e mice

1

Murine Tumor Implantation and Immunotherapy

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FVB and 129-E mice were purchased from Charles River Laboratories. Tumor cells were implanted in 7–9-week-old mice subcutaneously at optimal doses as indicated in figure legends. Tumor length and width were measured by caliper and the volume calculated by the equation: 0.4 × length × width2. For bioluminescence studies, images were obtained with the IVIS Spectrum imager after intraperitoneal (IP) injection of d-luciferin (150 mg/kg). For immunotherapy studies, mice bearing ~ 50 mm3 tumors were randomized and treated with 200 μg of anti-PD-1 antibody (BioXcell, Clone RMP1-14) or isotype control via IP injections three times weekly for four doses. Mice were housed in pathogen-free facilities at UCLA and all procedures were approved by the UCLA Animal Research Committee.
Salehi-Rad R., Li R., Tran L.M., Lim R.J., Abascal J., Momcilovic M., Park S.J., Ong S.L., Shabihkhani M., Huang Z.L., Paul M., Shackelford D.B., Krysan K., Liu B, & Dubinett S.M. (2021). Novel Kras-mutant murine models of non-small cell lung cancer possessing co-occurring oncogenic mutations and increased tumor mutational burden. Cancer Immunology, Immunotherapy, 70(8), 2389-2400.
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2

Murine Tumor Xenograft Model

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FVB and 129-E mice were purchased from Charles River Laboratories. Tumor cells were implanted in 7–9-week-old mice subcutaneously at optimal doses as indicated in figure legends. Tumor length and width were measured by caliper and the volume calculated by the equation: 0.4 × length × width2. For bioluminescence studies, images were obtained with the IVIS Spectrum imager after intraperitoneal (IP) injection of D-luciferin (150 mg/kg). For immunotherapy studies, mice bearing ∼ 50 mm3 tumors were randomized and treated with 200 μg of anti-PD-1 antibody (BioXcell, Clone RMP1–14) or isotype control via IP injections three times weekly for four doses. Mice were housed in pathogen-free facilities at UCLA and all procedures were approved by the UCLA Animal Research Committee.
Salehi-Rad R., Li R., Tran L.M., Lim R.J., Abascal J., Momcilovic M., Park S.J., Ong S.L., Shabihkhani M., Huang Z.L., Paul M., Shackelford D.B., Krysan K., Liu B, & Dubinett S.M. (2021). Novel Kras-mutant murine models of non-small cell lung cancer possessing co-occurring oncogenic mutations and increased tumor mutational burden. Cancer Immunology, Immunotherapy, 70(8), 2389-2400.
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