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Rat anti mouse ly 6g clone 1a8

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The Rat anti-mouse Ly-6G (clone 1A8) is a monoclonal antibody that specifically binds to the Ly-6G antigen expressed on the surface of mouse granulocytes, particularly neutrophils. It can be used for the identification and enumeration of these cell types in flow cytometric analysis.

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Lab products found in correlation

Rat anti mouse f4 80 clone ci a3 1, by BioXCell (2 mentions) Alexa fluor anti rabbit, by Thermo Fisher Scientific (1 mentions) Tcs sp5, by Leica (1 mentions)

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Clone 1A8

5 protocols using rat anti mouse ly 6g clone 1a8

1

Immunofluorescent Profiling of Lung Tissue

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Precision cut lung slices were incubated with primary antibodies: rat anti-mouse Ly-6G (clone 1A8, Bio X-Cell), hamster anti-mouse CD31 (2H8, Life Technologies), rat anti-mouse S100A9 (MU14-2A5, Hycult Biotech), polyclonal rabbit anti-mouse Ci-H3 (ABCAM) diluted in PBS-BSA-Triton-for 48h at 4°C. The sections were washed in PBS and incubated with low species cross-reactive fluorophore-conjugated secondary antibodies (Cy3 / Cy5 anti-rat, anti-hamster, Jackson; AlexaFluor anti-rabbit Life Technologies) for two hours. Sections were washed in PBS-BSA-Triton, then PBS and post-fixed with 4% PFA for 5 min. After a subsequent rinsing step, the slices were transferred into a 24-well imaging plate (IBIDI), covered with buffered Mowiol 4–88, pH 8.5 (Sigma-Aldrich) then coverslipped. Sections were evaluated by using a confocal laser-scanning microscope (TCS-SP5, Leica).
Karadjian G., Fercoq F., Pionnier N., Vallarino-Lhermitte N., Lefoulon E., Nieguitsila A., Specht S., Carlin L.M, & Martin C. (2017). Migratory phase of Litomosoides sigmodontis filarial infective larvae is associated with pathology and transient increase of S100A9 expressing neutrophils in the lung. PLoS Neglected Tropical Diseases, 11(5), e0005596.
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2

Monocyte and Neutrophil Depletion Protocol

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Antibodies were administered intraperitoneally according to the schedule described in Fig. 2a. Rat anti-mouse Ly-6G (clone 1A8; Bio X Cell) antibodies were administered at 200 μg/mouse 2 days before U-grafts implantation, and then every other day at 100 μg/mouse. Rat anti-mouse F4/80 (clone CI:A3-1; Bio X Cell) antibodies were administered at 400 μg/mouse 2 days before U-grafts implantation, and then every day at 200 μg/mouse. Daily injection of rat IgG (200 μg/mouse) served as control. Monocyte and neutrophil depletion was confirmed in blood samples by flow cytometry.
Lin R.Z., Lee C.N., Moreno-Luna R., Neumeyer J., Piekarski B., Zhou P., Moses M.A., Sachdev M., Pu W.T., Emani S, & Melero-Martin J.M. (2017). Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks. Nature biomedical engineering, 1, 0081.
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3

Ly6G/Ly6C (Gr-1) Depletion in Transplant

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Rat anti-mouse Ly6G/Ly6C (Gr-1) clone RB6-8C5 and rat anti-mouse Ly6G clone 1A8 were obtained from BioXcell. Then, 500 μg of RB6-8C5 was administered intraperitoneally on days 0, 2, 4, 6, and 8 after transplantation prior to analysis of allograft histopathology. Then, 500 μg of 1A8 was administered intraperitoneally on days −1, 3, 5, 7, 8, and 9 prior to analysis on day 10.
Lendermon E.A., Dodd-o J.M., Coon T.A., Wang X., Ensor C.R., Cardenes N., Koodray C.L., Heusey H.L., Bennewitz M.F., Sundd P., Bullock G.C., Popescu I., Guo L., O’Donnell C.P., Rojas M, & McDyer J.F. (2018). Azithromycin Fails to Prevent Accelerated Airway Obliteration in T-bet−/− Mouse Lung Allograft Recipients. Transplantation proceedings, 50(5), 1566-1574.
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4

Macrophage and Neutrophil Depletion Protocols

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Depletion of resident macrophages in either Cd169DTR or Fut7−/−; Cd169DTR mice was performed by intraperitoneal injection of DT (10 µg/kg; Sigma) at days −7, −5, and −2 before analysis. Alternatively, macrophages were depleted by a single intravenous injection of clodronate-loaded liposomes (100 µl, intravenously) 5 d before analysis. For neutrophil depletion, mice were injected subcutaneously with 3 µg/g mouse body weight of either a neutrophil-depleting antibody (rat anti-mouse Ly6G, clone 1A8; BioXcell) or control rat IgG (I4131; Sigma) for two consecutive days. Alternatively, Mrp8CRE; iDTR mice were injected subcutaneously with DT (10 ng/g mouse body weight) for three consecutive days before analysis.
Casanova-Acebes M., Nicolás-Ávila J.A., Li J.L., García-Silva S., Balachander A., Rubio-Ponce A., Weiss L.A., Adrover J.M., Burrows K., A-González N., Ballesteros I., Devi S., Quintana J.A., Crainiciuc G., Leiva M., Gunzer M., Weber C., Nagasawa T., Soehnlein O., Merad M., Mortha A., Ng L.G., Peinado H, & Hidalgo A. (2018). Neutrophils instruct homeostatic and pathological states in naive tissues. The Journal of Experimental Medicine, 215(11), 2778-2795.
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5

Monocyte and Neutrophil Depletion Protocol

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Antibodies were administered intraperitoneally according to the schedule described in Fig. 2a. Rat anti-mouse Ly-6G (clone 1A8; Bio X Cell) antibodies were administered at 200 μg/mouse 2 days before U-grafts implantation, and then every other day at 100 μg/mouse. Rat anti-mouse F4/80 (clone CI:A3-1; Bio X Cell) antibodies were administered at 400 μg/mouse 2 days before U-grafts implantation, and then every day at 200 μg/mouse. Daily injection of rat IgG (200 μg/mouse) served as control. Monocyte and neutrophil depletion was confirmed in blood samples by flow cytometry.
Lin R.Z., Lee C.N., Moreno-Luna R., Neumeyer J., Piekarski B., Zhou P., Moses M.A., Sachdev M., Pu W.T., Emani S, & Melero-Martin J.M. (2017). Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks. Nature biomedical engineering, 1, 0081.
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