Methanol lc ms chromasolv

The chemicals, BPA standard, NP standard, CAF standard, HHCB standard, AHTN standard, CBZ standard, methanol LC-MS CHROMASOLV^®, and dichloromethane LC-MS CHROMASOLV^®, hydrochloric acid (HCl), sodium hydroxide (NaOH), and standard pH buffers (for pH 4 and 7) were obtained from Sigma-Aldrich, Johannesburg, South Africa. All chemicals were used without further purification.

L-Glutathione (reduced), glutathione oxidized, L-methionine, trans-2-hexenal, acetonitrile, sodium borohydride (NaBH₄), ethanol, N-(tert-butoxycarbonyl)-L-cysteine (Boc-Cys-OH), triethylamine, sodium sulfate, triethylsilane, trifluoroacetic acid, 4-methylpent-3-en-2-one (mesityl oxide), mesityl oxide-d₁₀, sodium carbonate, acetic acid, hydrochloric acid, (+)-catechin, (−)-epicatechin, (−)-epicatechin gallate, procyanidin B1 and procyanidn B2 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Diethyl ether, pyridine, dichloromethane, 1,4-dioxane, pentane and ethyl acetate were obtained from Merck (Darmstadt, Germany). Formic acid (LC-MS, Fluka), methanol (LC-MS, Chromasolv, Sigma-Aldrich) and ultra-pure water of Milli Q gradient (EMD Millipore, Billerica, MA, USA) were used for chromatography. For grape grinding under cryogenic conditions (−196 °C) a M20 mill from IKA (Staufen, Germany) was used.

Drugs: Methiopropamine hydrochloride (HCl) (mol. wt. 191.7) was purchased from Cayman Chemicals (Middlesex, UK) and (+)methamphetamine HCl (mol. wt. 185.7) was purchased from Chiron AS (Trondheim, Norway). Chemicals: 5-HT HCl, L-NE HCl, 5-HIAA HCl, 3-MT HCl, ephedrine-D₃, sodium fluoride, methanol (LC-MS Chromasolv^®), ammonium formate, benztropine mesylate, reboxetine mesylate hydrate, fluoxetine HCl and adenosine 5′-triphosphate disodium salt hydrate were purchased from Sigma-Aldrich (Oslo, Norway). DA HCl and DOPAC were purchased from Fluka (Buchs, Switzerland). DA-¹³C₆ HCl, 5-HT-D₄ HCl, 5-HIAA-D₅, methamphetamine-¹³C₆ and nor-methiopropamine were purchased from Chiron AS. Di-sodiumtetraborate-10-hydrate and ethyl acetate were purchased from Chemi-Teknik AS (Oslo, Norway). Acetic acid and formic acid were purchased from VWR International AS (Oslo, Norway). Sodium hydroxide and n-heptane were purchased from Merck Millipore (Oslo, Norway). Dihydroxyphenylethylamine 3,4-[ring-2,5,6-³H]-(DA) ([³H]DA), NE HCl DL-[7-³H(N)] ([³H]NE), 5-HT [³H(G)] ([³H]5-HT) and UltimaGold^TM liquid scintillation cocktail were purchased form from PerkinElmer Inc. (Oslo, Norway). HEPES was purchased from Thermo Fischer Scientific (Oslo, Norway) and sodium heparin was purchased from Leo Pharma (Oslo, Norway).