The agomir and antagomir for miR-128-3p (pre-miR-128-3p and anti-miR-128-3p) as well as their negative control (pre-miR-con and anti-miR-con) were obtained from GenePharma (Shanghai, China). Lipofectamine 3000 (Invitrogen, Foster City, CA, USA) was applied to transfect miRNAs into A375 and M14 cells according to manufacturers’ protocols. The transfected efficiency was evaluated with use of qRT-PCR, with 48 h after transfection being the best time point for subsequent examinations.
Anti mir con
Manufactured by GenePharma
Sourced in China
Anti-miR-con is a laboratory tool used to inhibit the function of microRNA (miRNA) molecules. It is designed to provide a controlled and consistent approach for researchers to investigate the role of specific miRNAs in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Mir con, by GenePharma (13 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (11 mentions)
Si con, by GenePharma (8 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (5 mentions)
Anti mir con, by Thermo Fisher Scientific (5 mentions)
Mir con, by Thermo Fisher Scientific (3 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (3 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (2 mentions)
Anti mir 128 3p, by GenePharma (1 mentions)
Anti mir 15b, by GenePharma (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Si uca1 1, by GenePharma (1 mentions)
Pcdna empty vector vector, by GenePharma (1 mentions)
Anti mir 29c, by Thermo Fisher Scientific (1 mentions)
Si neat1, by GenePharma (1 mentions)
Si creb5, by GenePharma (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Pcdna, by GenePharma (1 mentions)
Lentiviral short hairpin rna, by Genechem (1 mentions)
Sh con, by Genechem (1 mentions)
21 protocols using anti mir con
1
miR-128-3p Transfection in Melanoma
2
Modulating miR-15b Expression Using Mimics and Inhibitors
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miR-15b mimics, miRNA mimic negative control (miRcon), miR-15b inhibitors (anti-miR-15b), and miRNA inhibitor negative control (anti-miR-con) were purchased from GenePharma (Shanghai, China). Synthetic miR-15b mimics, miR-con, miR-15b inhibitors, and anti-miR-con were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Small interfering RNA (siRNA) vectors targeting BACE1 were purchased from GenePharma (Shanghai, China). Transfection was conducted using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions.
3
Overexpression of Flot2 and miR-802 Modulation
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Overexpression Flot2 plasmid pcDNA3.1-Flot2, miR-802 mimics, miR-802 inhibitor and their control miRNA oligonucleotides (miR-Con, anti-miR-Con) were acquired from GenePharm (Shanghai, China) and transfected into the cells with Lipofectamine™ 2000 transfection reagent (Invitrogen) according to the manufacturer’s instructions. In cell transfection, cells were seeded in six-well plates and cultured until 50–70% confluency was reached in 1 day.
4
Targeting UCA1 lncRNA in Renal Cell Carcinoma
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siRNAs targeting UCA1 (siUCA1-1) and siUCA-2 and its scrambled control (siCon), miR129 mimic and its scrambled control (miR-Con), miR129 inhibitor (anti-miR129), and anti-miR-Con were purchased from GenePharma (Shanghai, China). Full-length sequences of UCA1 were amplified by polymerase chain reaction (PCR) and inserted into pcDNA3.1 vector (Thermo Fisher Scientific) to gain the UCA1-overexpression plasmid. All oligonucleotides or plasmids were transfected into RCC cells using Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. The target sequences of siUCA1 were siUCA1-1 (5′-GGACAACAGTACACGCATA-3′) and siUCA1-2 (5′-TCTTTGTCTCCTGGATTAAC-3′).
5
Investigating XIST and miR-29c in NPCs
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siRNAs against XIST (si-XIST-1, si-XIST-2), siRNA control (si-con), pcDNA-XIST, pcDNA empty vector (vector), miR-29c mimic (miR-29c), miRNA control (miR-con), miR-29c inhibitor (anti-miR-29c), and control inhibitor (anti-miR-con) were purchased from GenePharma Co., Ltd. (Shanghai, China). NPC cells were seeded into 6-well plates and cultured with complete medium without antibiotics at least 24 h prior to transfection. Then, cells were transiently transfected with siRNAs, miRNAs, or pcDNAs, or cotransfected with si-XIST and anti-miR-29c or anti-miR-con using Lipofectamine™ 2000 (Invitrogen). The cells were harvested at different time points for analysis post-transfection.
6
Investigating miR-194 and NEAT1 in Osteosarcoma
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The scrambled control miR (miR-con), miR-194 mimics (UGUA ACAGCAACUCCAUGUGGA), scrambled control anti-miR (anti-miR-con), and anti-miR-194 (UCCACAUGGAGUUGCU GUUACA) were synthesized by Genepharma (Shanghai, China). NEAT1 (GenBank #EF177379.1) was amplified from the cDNA of MG63 and U2OS cells and cloned into the pcDNA3.1 plasmid, which was named pcDNA-NEAT1. The siRNA sequence targeting NEAT1 was 5′-GUGAGAAGUUGCUUAGAAACUU UCC-3′ (si-NEAT1). si-NEAT1 and negative control siRNA (sicon) were obtained from GenePharma. Cells were transfected with plasmid or nucleotide sequences using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.
7
Investigating circVAPA and CREB5 roles
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CircVAPA small interfering RNA (si-circVAPA), si-CREB5 and their negative control (si-con), miR-125a mimics (miR-125a), miR-125a inhibitor (anti-miR-125a) and their negative controls (miR-con and anti-miR-con) were provided by GenePharma (Shanghai, China). Moreover, pcDNA3.1-circVAPA (circVAPA), pcDNA3.1-CREB5 (CREB5) and pcDNA 3.1 empty vector (vector) were obtained from Genecreat (Wuhan, China). The oligonucleotide and vector were transfected in HCT116 and LOVO cells using Lipofectamine 3000 reagents (Invitrogen)based on the instruction guidelines.
8
Modulating SNHG12 and miR-148a in Cells
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SNHG12 small interfering RNA and overexpression plasmid (si-SNHG12 and SNHG12) or their negative controls (si-con and pcDNA), miR-148a mimic and inhibitor (miR-148a and anti-miR-148a) or their negative controls (miR-con and anti-miR-con), CDK1 overexpression plasmid (CDK1) and its negative control (pcDNA) were designed and synthesized by GenePharma (Shanghai, China). Lentiviral short hairpin RNA targeting SNHG12 (sh-SNHG12) and its negative control (sh-con) were constructed by Genechem (Shanghai, China). Cells were transfected with indicated plasmids using Lipofectamine 3000 (Invitrogen).
9
Investigating miR-195 and OSBP in Cancer Cell Lines
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Human SW872 and 93T449 cells (American Type Culture Collection, ATCC, Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle Medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum and 1% antibiotics (TaKaRa, Dalian, China). All cells were maintained in a 37°C, 5% CO2 incubator. miR-195 (GenePharma, Shanghai, China), and its corresponding scrambled oligonucleotide sequence (miR-con, GenePharma), anti-miR-195 (GenePharma) and its corresponding scrambled oligonucleotide sequence (anti-miR-con, GenePharma) were transfected into cells using Dharma FECT 4 transfection reagent (Dharmacon, Brebières, France), referring to the protocols of manufacturers. The full sequences of OSBP were inserted into the pcDNA vector (Promega, Southampton, UK) to construct OSBP overexpression plasmid (OSBP), and nontarget vector (pcDNA) was used as a negative control.
10
Podocyte Stress Response to High Glucose
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Mice podocytes were obtained from BeNa Culture Collection (BNCC, Suzhou, China) and cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Gibco), 100 U/mL penicillin and 0.1 mg/mL streptomycin at 37°C in 5% CO2. Meanwhile, different concentrations (0, 10, 20, 30 and 40 mM) of HG were used to treat the podocytes at different times (0, 12, 24 and 48 h) to determine the gene expression pattern and confirm the optimal concentration and time. A miR-203-3p mimic and inhibitor (miR-203-3p and anti-miR-203-3p) or their negative controls (miR-con and anti-miR-con), as well as a Semaphorin 3A (Sema3A) overexpression plasmid and its negative control (pcDNA) were synthesized by GenePharma (Shanghai, China). All plasmid oligonucleotides were transfected into podocytes using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).
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