Cell culture LNCaP and U937 were purchased from ATCC (Milan, Italy). LNCaP were grown in Roswell Park Memorial Institute culture medium (RPMI; EuroClone, Milan, Italy), supplemented with 10% heat-inactivated foetal bovine serum (FBS; Sigma-Aldrich, Schnellendorf, Germany), antimicrobials (100 U/mL penicillin, 100 µg/mL streptomycin, 250 ng/mL amphotericin-B), 2 mM L-glutamine (EuroClone, Milan, Italy), and 1% essential amino acids solution (MEM; EuroClone, Milan, Italy). U937 were cultured in Dulbecco’s Modified Eagle Medium (DMEM; EuroClone, Milan, Italy), supplemented with 10% heat-inactivated foetal bovine serum (FBS; Sigma-Aldrich, Schnellendorf, Germany), antimicrobials (100 U/mL penicillin, 100 µg/mL streptomycin, 250 ng/mL amphotericin-B), 2 mM L-glutamine (EuroClone). Cells have grown in standard incubator conditions at 37 °C and 5% CO2.
Lncap
Manufactured by Euroclone
Sourced in Italy
The LNCaP cell line is a well-established human prostate adenocarcinoma cell line derived from a lymph node metastasis. It is commonly used in cancer research, particularly for the study of prostate cancer.
Automatically generated - may contain errors
Lab products found in correlation
Lncap, by American Type Culture Collection (2 mentions)
L glutamine, by Euroclone (2 mentions)
Du145, by Euroclone (2 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Roswell park memorial institute (rpmi), by Euroclone (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Euroclone (1 mentions)
Lncap, by Thermo Fisher Scientific (1 mentions)
Du145, by Thermo Fisher Scientific (1 mentions)
Egm singlequot, by Lonza (1 mentions)
Human umbilical vein endothelial cells (huvec), by Lonza (1 mentions)
Fetal bovine serum (fbs), by Euroclone (1 mentions)
Glutamine, by Euroclone (1 mentions)
Penicillin, by Euroclone (1 mentions)
Streptomycin, by Euroclone (1 mentions)
3 protocols using lncap
1
Culturing LNCaP and U937 Cell Lines
2
Culturing PC Cell Lines
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Human PC cell lines, LNCaP, PC3 and DU145, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were authenticated using Short Tandem Repeat analysis as described in ANSI Standard (ASN-0002) by ATCC Standards Development Organization (SDO). LNCaP, PC3 and DU145 cells were maintained in RPMI-1640 medium (EuroClone, Milano, Italy) supplemented with 10% (PC3) and 5% (LNCaP and DU145) fetal bovine serum (FBS) (Gibco, ThermoFisher Scientific), glutamine (1 mmol/l) and antibiotics (100 IU/ml penicillin G) and cultured at 37 °C in humidified atmosphere of 5% CO2.
3
Culturing Cancer and Immune Cells
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The human prostate cancer (PCa) cell lines PC-3, DU-145, LNCaP (all purchased by ATCC) were maintained in in RPMI 1640 medium, supplemented with 10% Fetal Bovine Serum (FBS), (Euroclone), 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone), at 37°C, 5% CO2. Cells were routinely screened for eventual mycoplasma contaminations. Conditioned media (CM) were collected following 72 hours of starvation in FBS free RMPI 1640 (Life Technologies), supplemented with 1% Glutamine (Euroclone) and 1% P/S (Euroclone), at 37°C, 5% CO2. CMs were used for NK cell polarization as detailed below.
Human umbilical vein endothelial cells (HUVEC, Lonza) were maintained in endothelial cell basal medium (EBM™, Lonza) supplemented with endothelial cell growth medium (EGM™ SingleQuots™, Lonza), 10% of FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone). HUVEs were used between the 3-5 passages.
The human monocytic THP-1 cell line (ATCC) was cultured in RPMI 1640 medium, supplemented with 10% FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone), at 37°C, 5% CO2. Differentiation of adherent THP-1 macrophages was obtained following 48 hours of treatments with phorbol-merystate-acetate (5 ng/mL, PMA, Sigma Aldrich), as in [29] (link).
Human umbilical vein endothelial cells (HUVEC, Lonza) were maintained in endothelial cell basal medium (EBM™, Lonza) supplemented with endothelial cell growth medium (EGM™ SingleQuots™, Lonza), 10% of FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone). HUVEs were used between the 3-5 passages.
The human monocytic THP-1 cell line (ATCC) was cultured in RPMI 1640 medium, supplemented with 10% FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone), at 37°C, 5% CO2. Differentiation of adherent THP-1 macrophages was obtained following 48 hours of treatments with phorbol-merystate-acetate (5 ng/mL, PMA, Sigma Aldrich), as in [29] (link).
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