Total rna extraction

Manufactured by Tiangen Biotech

Sourced in China

The Total RNA Extraction Kit is a laboratory equipment designed for the rapid and efficient extraction of total RNA from a variety of biological samples, including cells, tissues, and microorganisms. It utilizes a guanidinium thiocyanate-phenol-chloroform extraction method to isolate high-quality RNA that can be used in various downstream applications, such as RT-PCR, Northern blotting, and RNA sequencing.

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Lab products found in correlation

High fat diet (hfd), by Beijing Huafukang Biotechnology (1 mentions) Oil red o staining kit, by Solarbio (1 mentions) Glutathione peroxidase gsh px assay kits test kits, by Nanjing Jiancheng (1 mentions) Cdna synthesis, by Tiangen Biotech (1 mentions)

2 protocols using total rna extraction

Nuciferine's Effects on Lipid Metabolism

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Nuciferine (C₁₉H₂₁NO₂; molecular weight, 295.38 Da; purity ≥ 98%) was purchased from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China). HFD (17.7% sucrose, 17.7% fructose, 19.4% protein, and 40% fat) was obtained from Beijing Huafukang Bioscience Co., Ltd. (Beijing, China). Triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), and glutathione peroxidase (GSH-Px) assay kits test kits were obtained from Nanjing Jiancheng Biological Engineering Institute (Nanjing, China). Oil Red O Staining Kit was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China). Total RNA extraction, first-strand cDNA reverse transcription, polymerase chain reaction (PCR) kits and primers were obtained from TianGen Biotechnology Co., Ltd. (Beijing, China). Rat interleukin (IL)-6, IL-1β, and tumor necrosis factor-alpha (TNF-α) enzyme-linked immunosorbent assay (ELISA) kits were obtained from Shanghai BlueGene Biotech Co., Ltd. (Shanghai, China).

Cui H., Li Y., Cao M., Liao J., Liu X., Miao J., Fu H., Song R., Wen W., Zhang Z, & Wang H. (2020). Untargeted Metabolomic Analysis of the Effects and Mechanism of Nuciferine Treatment on Rats With Nonalcoholic Fatty Liver Disease. Frontiers in Pharmacology, 11, 858.

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Total RNA Extraction and cDNA Synthesis

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The steps of total RNA extraction (Tiangen Biotech (Beijing) Co., Ltd) and cDNA synthesis (Takara) were performed according to the manufacturer's instructions. Primers used in quantitative real-time PCR are listed in Table 1 (Wang et al., 2020 (link)).

Dong C.L., Qin Y., Ma J.X., Cui W.Q., Chen X.R., Hou L.Y., Chen X.Y., God’spower B.O., Eliphaz N., Qin J.J., Guo W.X., Ding W.Y, & Li Y.H. (2021). The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder. Frontiers in Pharmacology, 11, 612478.

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