5637, T24 and SW780 cell viability was measured using the CCK-8 Cell Proliferation/Viability Assay Kit (Dojindo, Japan) as described previously [17 (link)]. Absorbance was calculated at 450 nm using an Elx800 Reader (Bio-Tek Instruments Inc., Winooski, VT, USA).
Cck 8 cell proliferation viability assay kit
Manufactured by Dojindo Laboratories
Sourced in Japan
The CCK-8 Cell Proliferation/Viability Assay Kit is a colorimetric assay that measures the metabolic activity of cells. It utilizes a water-soluble tetrazolium salt that is reduced by dehydrogenases in viable cells, producing a colored formazan dye. The intensity of the color is proportional to the number of living cells, enabling quantification of cell proliferation or viability.
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Lab products found in correlation
4 protocols using cck 8 cell proliferation viability assay kit
1
Cell Viability Assay Protocol
2
Cell Viability Assay Protocol
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MKN-28 and MGC-803 cells were seeded in a 96-well plate for 24, 48 and 72 h transfected with corresponding plasmids. Cell viability was detected using the CCK-8 Cell Proliferation/Viability Assay Kit (Dojindo, Japan). Absorbance was recorded at 450 nm using Elx800 Reader (Bio-Tek Instruments Inc., Winooski, VT, USA).
3
Evaluating Cell Viability and Proliferation
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MCF-7 and MDA-MB-231 cells (1 × 104) were seeded in the 96-well plate for 24, 48 and 72 h transfected with si-0004771 or si-circRNA-NC, miR-653 mimics or miR-NC, and sh-ZEB2 or sh-NC. Cells viability was measured using cell counting kit-8 (CCK-8) Cell Proliferation/Viability Assay Kit (Dojindo Japan). Absorbance was recorded at 450 nm using Elx800 Reader (Bio-Tek Instruments Inc., Winooski, VT, U.S.A.).
4
Cell Viability Measurement Using CCK-8 Assay
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After treatment with different conditions for 24, 48 or 72 h, cell viability was measured using the CCK-8 Cell Proliferation/Viability Assay Kit (Dojindo, Japan). Absorbance was recorded at 450 nm using an Elx800 Reader (Bio-Tek Instruments Inc., Winooski, VT, USA).
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