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Ab45766

Manufactured by Abcam
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Sourced in United States

Ab45766 is a laboratory equipment product. It is designed to perform a specific core function in the laboratory setting. The details of its use and intended application are not provided in this factual and unbiased description.

Automatically generated - may contain errors

Lab products found in correlation

Ab70335, by Abcam (2 mentions) Ab155102, by Abcam (1 mentions) Ab66340, by Abcam (1 mentions) Ab38007, by Abcam (1 mentions) Ab32572, by Abcam (1 mentions) Protein a g magnetic bead, by Thermo Fisher Scientific (1 mentions) Ab113205, by Abcam (1 mentions) Thermo scientific rip kit, by Thermo Fisher Scientific (1 mentions) Ab26096, by Abcam (1 mentions) Ab92513, by Abcam (1 mentions) Ab9110, by Abcam (1 mentions) Cell lysis buffer, by Promega (1 mentions) Sc 130300, by Santa Cruz Biotechnology (1 mentions) Sc 10809, by Santa Cruz Biotechnology (1 mentions) Sc 33 614, by Santa Cruz Biotechnology (1 mentions)

5 protocols using ab45766

1

RIP Assay for Protein-RNA Interactions

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Cells were cross-linked at 254 nm by ultraviolet light, and RIP assay was performed according to the instructions of Magna RIPTM Kit (Millipore, Bedford, MA, USA), with antibodies specific for CNBP (ab48027), ENO1 (ab155102), NAP1L1 (ab33076), Flag (ab45766, Abcam lnc.), HDGF (A10435), NONO (A5282), SFPQ (A0958, ABclonal). Co-precipitated RNAs were detected by RT-PCR or real-time qRT-PCR with specific primers (Additional file 1: Table S2).
Yang F., Hu A., Li D., Wang J., Guo Y., Liu Y., Li H., Chen Y., Wang X., Huang K., Zheng L, & Tong Q. (2019). Circ-HuR suppresses HuR expression and gastric cancer progression by inhibiting CNBP transactivation. Molecular Cancer, 18, 158.
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2

Comprehensive Protein Analysis Protocol

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Western blot analysis was performed as described before with antibodies specific for β‐actin (ab125402), β‐catenin (ab32572), RBM15 (ab244374), ENO1 (ab155102), GPI (ab66340), PGK1 (ab38007), Flag (ab45766, Abcam, USA), ALDOA (sc‐390733) and IGF2BP1 (sc‐166344, Santa Cruz Biotechnology, USA).
Yang F., Liu Y., Xiao J., Li B., Chen Y., Hu A., Zeng J., Liu Z, & Liu H. (2022). Circ‐CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15. Cell Proliferation, 56(1), e13344.
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3

Co-Immunoprecipitation Assay Protocol

Cited 1 time
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Co-IP assay was performed as reported previously [52 (link)], using antibodies for NONO (ab70335), HA (ab32572), or FLAG (ab45766, Abcam Inc.). After releasing from bead-bound complex, protein levels were measured by western blotting.
Li D., Chen Y., Mei H., Jiao W., Song H., Ye L., Fang E., Wang X., Yang F., Huang K., Zheng L, & Tong Q. (2018). Ets-1 promoter-associated noncoding RNA regulates the NONO/ERG/Ets-1 axis to drive gastric cancer progression. Oncogene, 37(35), 4871-4886.
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4

RNA-Protein Interactions Analysis

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RNA immunoprecipitation (RIP) assays were performed according to the instructions of the Thermo Scientific RIP Kit (Thermo, Waltham, MA, USA) using specific antibodies against HNRNPK (11426-1-AP, Proteintech, China), ILF2 (ab113205, Abcam, UK), Flag (ab45766, Abcam, UK), or IgG (30000-0-AP, Proteintech, China) and Protein A/G magnetic beads (Life Technologies, USA) for RNA immunoprecipitation. Input and coprecipitated RNA were detected via qRT‒PCR analysis.
Xi Z., Huang H., Hu J., Yu Y., Ma X., Xu M., Ming J., Li L., Zhang H., Chen H, & Huang T. (2024). LINC00571 drives tricarboxylic acid cycle metabolism in triple-negative breast cancer through HNRNPK/ILF2/IDH2 axis. Journal of Experimental & Clinical Cancer Research : CR, 43, 22.
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5

Western Blotting of Cancer Proteins

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Protein of cancer cells or tissues was prepared using 1× cell lysis buffer (Promega). Western blotting was performed as previously reported [12 (link), 13 (link), 49 (link)–51 (link)], with antibodies specific for NONO (ab70335), ERG (ab92513), Ets-1 (ab26096), FLAG (ab45766), HA (ab9110, Abcam Inc., Cambridge, MA), histone H3 (sc-10809), GST (sc-33614), and β-actin (sc-130300, Santa Cruz Biotechnology, Santa Cruz, CA).
Li D., Chen Y., Mei H., Jiao W., Song H., Ye L., Fang E., Wang X., Yang F., Huang K., Zheng L, & Tong Q. (2018). Ets-1 promoter-associated noncoding RNA regulates the NONO/ERG/Ets-1 axis to drive gastric cancer progression. Oncogene, 37(35), 4871-4886.
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