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3 protocols using 3 phenylphenol

1

Fiber Chemical Composition Analysis

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Chemical composition of the fiber was determined with a gravimetric method as described in De Prez et al. [11 ] and is based on procedures described by Bledzki et al. [20 ] and Ramadevi et al. [21 ]. The gravimetric method enables us to determine cellulose, hemicellulose and lignin content of the fiber. Pectin content was determined spectrophotometrically by using 3-phenylphenol (90%, Acros Organics) as described in De Prez et al. [11 ] and is based on methods described by [[22] (link), [23] (link), [24] ]. Samples were analyzed in triplicate and assays were repeated twice.
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2

Antimicrobial Assessment of D-Limonene

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D-limonene (97%) were purchased from Alfa Aesar (Tewksbury, MA USA). Plate count agar and Tryptone soy broth were purchased from Oxoid (Basingstoke, Hampshire, United Kingdom). Ethanol (95%) was purchased from Echo Chemical Co. (Miaoli County, Taiwan). Acetone were purchased from Seedchem (Box Hill, VIC, Australia). Sulfuric acid was purchased from Aencore Chemical Co. (Box Hill, VIC, Australia). Sodium chloride, disodium hydrogen phosphate, sodium hydroxide, and sodium tetraborate were purchased from Showa Chemical Industry Co. (Minato-ku, Tokyo, Japan). Potassium dihydrogen phosphate were purchased from Katayama Chemical Industries Co. (Hyogo, Japan). Propylene glycol were purchased from JT Baker (Phillipsburg, NJ, USA). Tween 80, glucose, phenol, and pectin (from citrus peel) were purchased from Sigma Aldrich (Quentin Fallavier, France). The 3-Phenylphenol and sodium alginate were purchased from Acros organics (Trenton, NJ, USA). Potassium hydrogen phthalate was purchased from Fullin Nihon Shiyaku (Taoyuan, Taiwan). Dichloran rose Bengal chloramphenicol agar was purchased from Becton, Dickinson and Co. (Washington, DC, USA).
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3

Characterization of Fruit and Vegetable Samples

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‘Jonagold’ apples (Malus domestica), yellow onions (Allium cepa), ‘Nerac’ carrots (Daucus carota) and ‘Hokkaido’ pumpkins (Cucurbita maxima) were bought at a local shop.
Technical ethanol (99%), technical acetone, Na2CO3 and NaOH pellets were bought from VWR (Leuven, Belgium). HCl, NaOH (0.1 M), H2SO4 (concentration ≥ 95% w/w) and disodium tetraborate decahydrate were obtained from Fisher Scientific (Merelbeke, Belgium). Rhamnose monohydrate, 3-phenylphenol and HNO3 were bought from Acros Organics (Geel, Belgium). H2SO4 (72% w/w) and NaOH (50% w/w) were obtained from Alfa Aesar (Kandel, Germany) and J.T. Baker (Gliwice, Poland), respectively. Galacturonic acid monohydrate and fucose were bought from Sigma-Aldrich (Diegem, Belgium), arabinose from Fluka Biochemika (Buchs, Switzerland), galactose from Merck (Darmstadt, Germany), glucose monohydrate from Riedel-de-Haën (Seelze, Germany), xylose from UCB (Leuven, Belgium) and mannose from Fluka Analytical (Buchs, Switzerland). The ultrapure water (organic free, 18.2 MΩ·cm resistance) was provided by a SimplicityTM 150 system. Unless otherwise mentioned, all chemicals used were of analytical grade.
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