Rabbit anti tfeb

Manufactured by Cell Signaling Technology

Rabbit anti-TFEB is a primary antibody that specifically recognizes the transcription factor EB (TFEB) protein. TFEB is a master regulator of lysosomal biogenesis and autophagy. This antibody can be used to detect and study the expression and localization of TFEB in various cell and tissue samples.

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Lab products found in correlation

Rabbit anti raptor, by Cell Signaling Technology (2 mentions) Mouse anti flag, by Merck Group (2 mentions) Rabbit anti mtor, by Cell Signaling Technology (2 mentions) Mouse anti gfp, by Thermo Fisher Scientific (1 mentions) Rabbit anti gapdh, by Thermo Fisher Scientific (1 mentions) Rabbit anti gfp, by Abcam (1 mentions) Mouse anti actin, by Santa Cruz Biotechnology (1 mentions) Rabbit anti lamp1, by Cell Signaling Technology (1 mentions) Rabbit anti lc3a b, by Cell Signaling Technology (1 mentions) Hoechst 33324, by Thermo Fisher Scientific (1 mentions) Mouse anti sqstm1 p62, by Novus Biologicals (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Tcs sp5 confocal microscope, by Leica (1 mentions) Okadaic acid, by Santa Cruz Biotechnology (1 mentions) Mouse anti gapdh, by Thermo Fisher Scientific (1 mentions) Torin 1, by Cayman Chemical (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Rabbit anti ulk1, by Cell Signaling Technology (1 mentions) Apilimod, by MedChemExpress (1 mentions) Fk506, by Cayman Chemical (1 mentions)

Close spelling variants of this product

Anti-TFEB (15 citations) Rabbit anti-TFEB (4240S; (2 citations) Rabbit polyclonal anti-TFEB (#4240), (2 citations)

5 protocols using rabbit anti tfeb

Investigating C9orf72 and TFEB Regulation

Cited 1 time

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C9orf72-Flag and C9orf72 shRNA constructs were described previously [27 (link)]. GFP-TFEB (38119, Shawn Ferguson, Yale University), pRK5-HA GST RagB (19301, David Sabatini, Whitehead Institute), pRK5-HA GST RagC (19304, David Sabatini, Whitehead Institute), myc-Raptor (1859, David Sabatini, Whitehead Institute), pRK5-HA GST RagB 99L (19303, David Sabatini, Whitehead Institute), and pRK5-HA GST RagC 75N (19305, David Sabatini, Whitehead Institute) were obtained from Addgene.
Antibodies used in the human cell studies include the following: mouse anti-Flag (Sigma), mouse anti-GFP, rabbit anti-GAPDH (ThermoFisher), mouse anti-actin (Santa Cruz), mouse anti-C9orf72 (Bio-Rad), mouse anti-C9orf72 (Proteintech), mouse anti-HA (Bethyl Lab), rabbit anti p-70S6K, rabbit anti p-p70S6K, rabbit anti-RagA, rabbit anti-RagC, rabbit anti-Raptor, rabbit anti-LAMP1, rabbit anti-DYKDDDDK, rabbit anti-pTFEB, rabbit anti-TFEB (Cell Signaling), mouse anti-TFEB (Mybiosources), and rabbit anti-GFP (Abcam).

Ji Y.J., Ugolino J., Zhang T., Lu J., Kim D, & Wang J. (2020). C9orf72/ALFA-1 controls TFEB/HLH-30-dependent metabolism through dynamic regulation of Rag GTPases. PLoS Genetics, 16(4), e1008738.

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Analyzing Autophagy Markers by Immunofluorescence

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Immunofluorescence was performed as described [65 (link)], using the following antibodies: goat anti-GFP FITC conjugated (1:400, Abcam), rabbit anti-LC3A/B (1:1000, Cell Signaling), mouse anti- SQSTM1/p62 (1:100, Novus Biological), rabbit anti-TFEB (1:100, Cell Signaling), rabbit anti-CathD (1:100, Calbiochem) and rabbit anti-Atg8a (1:200, Abcam). Anti-rabbit and anti-mouse Alexa Fluor secondary antibodies (Invitrogen) were used at 1:1000. Nuclei were visualized by staining the DNA with DAPI or Hoechst 33324 (Invitrogen). Images were acquired using a Leica TCS SP5 confocal microscope. Live cell images were obtained as previously described [18 ].

Cai W., Wei Y., Jarnik M., Reich J, & Lilly M.A. (2016). The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lysosome Function. PLoS Genetics, 12(5), e1006036.

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Antibody Validation and Signaling Pathway Analysis

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The following antibodies were used: rabbit anti-p70S6K (Cell Signaling Technologies; #2708), rabbit anti-p-p70S6K (Thr389) (Cell Signaling Technologies; #9205), rabbit anti-Akt (Cell Signaling Technologies; #9272), rabbit anti-p-Akt (Thr308) (Cell Signaling Technologies; #13038), rabbit anti-p-Akt (Ser473) (Cell Signaling Technologies; #4051), rabbit anti-ULK1 (Cell Signaling Technologies; #8054), rabbit anti-p-ULK1 (Ser757) (Cell Signaling Technologies; #6888), rabbit anti-TFEB (Cell Signaling Technologies; #4240), rabbit anti-p-TFEB (Ser211) (Cell Signaling Technologies; #37681), rabbit anti-4E-BP1 (Cell Signaling Technologies; #9644), rabbit anti-p-4E-BP1 (Thr37/46) (Cell Signaling Technologies; #2855), rabbit anti-mTOR (Cell Signaling Technologies; #2983), rabbit anti-Raptor (Cell Signaling Technologies; #2280), rabbit anti-HA (Cell Signaling Technologies; #3724), mouse anti-α-tubulin (Thermo; 236-10501), mouse anti-GAPDH (Thermo; AM4300), mouse anti-DYKDDDDK (WAKO; clone 1E6), mouse anti-LAMP1 (Development Studies Hybridoma Bank; H4A3), mouse anti-PIKfyve (Development Studies Hybridoma Bank; 3C9), rabbit anti-TFE3 (Sigma; HPA023881), mouse anti-PP2A-Cα/β (Santa Cruz; sc-80665), and rabbit anti-PPP2CA (Proteintech; 13482-1-AP). Apilimod was from MedChem Express. Okadaic acid was from Santa Cruz. Torin1, YM201636, VPS34-IN1, and FK-506 were from Cayman Chemical.

Hasegawa J., Tokuda E., Yao Y., Sasaki T., Inoki K, & Weisman L.S. (2022). PP2A-dependent TFEB activation is blocked by PIKfyve-induced mTORC1 activity. Molecular Biology of the Cell, 33(3), ar26.

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Comprehensive Immunostaining and Immunoblot Protocol

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Primary antibodies used for immunostaining and immunoblot included rabbit anti-MAP2 (CST, #4542), rabbit anti-DARPP-32 (19A3) (CST, #2306), rabbit anti-GABA (Sigma-Aldrich, A2052), mouse anti-NCAM1 (ERIC) (Santa Cruz, sc-106), rabbit anti-NEUN (Millipore, ABN78), mouse anti-ACTL6B (Antibodies Incorporated, 75-311), mouse anti-tubulin β III (Covance, MMS-435P), rabbit anti-tubulin β III (Covance, PRB-435P-100), rabbit anti-RCAN1/DSCR1 (Sigma-Aldrich, D6694), rabbit anti-pan-Calcineurin A (Cell signaling, 2614), rabbit anti-TFEB (Cell signaling, 4240), rabbit anti-phosphor-TFEB (Ser142) (Millipore, ABE1971), rabbit anti-phosphor-TFEB (Ser211) (Cell signaling, 37681), rabbit anti-p62/SQSTM1 (Abcam, ab109012), mouse anti-Flag (Sigma-Aldrich, F1804), rabbit anti-GAPDH (Santa Cruz, sc-32233) antibodies. The secondary antibodies included goat anti-mouse, rabbit IgG (H+L) HRP secondary antibody, and goat anti-rabbit, mouse, rat, or chicken IgG conjugated with Alexa-488, −594, or −647 (Thermo Fisher Scientific).

Lee S.W., Oh Y.M., Victor M.B., Strunilin I., Chen S., Dahiya S., Dolle R.E., Pak S.C., Silverman G.A., Perlmutter D.H, & Yoo A.S. (2023). Longitudinal modeling of human neuronal aging identifies RCAN1-TFEB pathway contributing to neurodegeneration of Huntington’s disease. Research Square.

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Immunofluorescence Microscopy of Organelles

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Mouse anti-LBPA (MABT837) antibody was from Millipore, Merk (diluted 1:100), and the rabbit anti-Lamp1 (#9091), mouse anti-Lamp1 (#15665), rabbit anti-mTOR (#2983), and rabbit anti-TFEB (#4240) were purchased from Cell Signalling (diluted 1:200, 1:100, 1:100, and 1:100, respectively).

Hartwig P, & Höglinger D. (2021). The Glucosylceramide Synthase Inhibitor PDMP Causes Lysosomal Lipid Accumulation and mTOR Inactivation. International Journal of Molecular Sciences, 22(13), 7065.

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