TEM images were obtained in a Talos F200S Microscope (Thermo Fisher Scientific) by using a 200-kV microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimens were prepared by dropping sample solutions (1 mg/mL in water/solvent) onto a 3-mm copper grid (lacey, 400 mesh) and leaving them to air-dry at room temperature. To determine the elemental composition of the ZIF-8 and Zn0.9Co0.1 (2Me-Im)2 specimen, EDX with two silicon drift detectors (SDD) was used. Counting time for X-ray spectra was 60 s.
Talos f200s microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Talos F200S microscope is a high-performance transmission electron microscope (TEM) designed for materials science research and analysis. It features a 200 kV accelerating voltage, a Schottky field emission gun, and advanced imaging and analytical capabilities. The Talos F200S provides users with a powerful tool for investigating the structure and composition of a wide range of materials at the nanoscale.
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Lab products found in correlation
Talos f200, by Thermo Fisher Scientific (3 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Sta7200, by Hitachi (1 mentions)
Nicolet nexus ftir spectrometer, by Thermo Fisher Scientific (1 mentions)
5848 microtester, by Instron (1 mentions)
Quanta 650 feg, by Thermo Fisher Scientific (1 mentions)
D8 advance, by Bruker (1 mentions)
Vector 22, by Brucker (1 mentions)
Nova nanosem 450, by Thermo Fisher Scientific (1 mentions)
Em uc7 ultramicrotome, by Leica (1 mentions)
Vertex 70, by Bruker (1 mentions)
Zetasizer nano zs system, by Malvern Panalytical (1 mentions)
Asap 2460, by Micromeritics (1 mentions)
Rf 5301pc, by Shimadzu (1 mentions)
Ultra performance liquid chromatography (uplc), by Waters Corporation (1 mentions)
Varioskan lux multimode, by Thermo Fisher Scientific (1 mentions)
Full wavelength microplate reader, by Thermo Fisher Scientific (1 mentions)
Bc 5000vet, by Mindray (1 mentions)
7 protocols using talos f200s microscope
1
Characterization of Metal-Organic Frameworks
2
Characterization of Coated Upconverting Nanoparticles
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Transmission electron microscopy (TEM) images were obtained with a Talos F200S Microscope (Thermo Fisher Scientific) using an accelerating voltage of 200 kV. The samples were prepared by placing a droplet of UCNP dispersions (1 mg/mL in water) onto a 3 mm copper grid (lacey, 400 mesh) and letting it dry under air at rt. The average particle size was determined from 2 to 3 micrographs (58 kx magnification) of approximately 500 particles. The area of the particles was automatically measured using a fixed threshold based on the image intensity histograms and the size distribution descriptors (Feretmax and Feretmin) were determined. The obtained diameters were plotted as histograms and fitted with a Gaussian curve. The mean (μ) and standard deviation (σx) of this curve were taken as the representative particle size for the respective sample.
FT-IR measurements of the coated particles were acquired on a Nicolet Nexus FT-IR spectrometer (Thermo Electron Corporation) using an ATR accessory. The spectra were recorded in a wavenumber range of 4000–400 cm−1.
Thermal gravimetric analysis (TGA) of the coated particles was performed using a Hitachi STA 7200 set-up with Sample Changer AS3. TGA experiments were performed over a temperature range of 30–600 °C under nitrogen atmosphere (200 mL/min), switching to synthetic air at 600 °C (200 mL/min), and using a heating rate of 10 K/min.
FT-IR measurements of the coated particles were acquired on a Nicolet Nexus FT-IR spectrometer (Thermo Electron Corporation) using an ATR accessory. The spectra were recorded in a wavenumber range of 4000–400 cm−1.
Thermal gravimetric analysis (TGA) of the coated particles was performed using a Hitachi STA 7200 set-up with Sample Changer AS3. TGA experiments were performed over a temperature range of 30–600 °C under nitrogen atmosphere (200 mL/min), switching to synthetic air at 600 °C (200 mL/min), and using a heating rate of 10 K/min.
3
Multimodal Characterization of Electrodes
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The phase constituents were determined by XRD (Bruker D8‐Advance) using a Cu Kα radiation. The morphologies of all electrodes were observed by field emission scanning electron microscopy (FEI Quanta 650 FEG). HAADF image and elemental mappings were performed on FEI Talos F200S microscope equipped with Super‐X EDX system, operated at 200 kV. In situ TEM was performed by a TEM‐STM in situ sample holder (Zep Tools Co. Ltd., China) on a FEI Talos F200S TEM. Microtensile tests were carried out under displacement control by an Instron 5848 Microtester. Elastic moduli was determined in the linear elastic region of the stress–strain curves.
4
Characterization of Functionalized Boron Nitride
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Fourier transform infrared spectroscopy (FT-IR) spectroscopy of h-BN, BNNP, Lys@BNNP and Glu@BNNP were recorded on a Vector-22 (Brucker, Germany) spectrometer. The spectral range was 400 to 4000 cm−1 with a resolution of 4 cm−1. All spectra were modified with carbon removal and baseline correction. Thermal gravimetric analysis (TGA) was conducted to evaluate the grafting content by using a SDT-TG Q600 thermogravimetric analyzer (TA Instruments, New Castle, DE, USA). The nitrogen flow rate was 100 mL min−1, the heating rate was 10 °C min−1, and the temperature scanning range was 100–700 °C. X-ray diffraction (XRD) was used to study the crystallinity degrees of the h-BN, BNNP, Lys@BNNP and Glu@BNNP. XRD data was recorded on a D8 Advance (Brook AXS, Germany) X-ray diffractometer. The elemental composition of Lys@BNNP and Glu@BNNP was analyzed by ESCALAB 250Xi (Thermofisher Scientific, Waltham, MA, USA) X-ray photoelectron spectroscopy (XPS). The morphologies of h-BN, BNNP, Lys@BNNP and Glu@BNNP were observed by a Nova Nano SEM 450 (FEI, Hillsboro, OR, USA) scanning electron microscope (SEM). The morphology of the samples was investigated using transmission electron microscopy (TEM) (FEI Talos F200S, Hillsboro, OR, USA). Elemental mapping analysis was conducted by using a FEI Talos F200S microscope.
5
Characterization of Yb(III) Adsorption on B. pumilus
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A high-resolution transmission electron microscope (TEM; Talos F200S, FEI, USA) equipped with Super-X X-ray spectroscopy operated at 200 kV was employed for high-resolution imaging and compositional analysis. The morphology of B. pumilus before or after Yb(III) adsorption was observed by TEM (80 (link)). The cell suspension was centrifuged and washed three times with NaCl solution (1.0 mmol L−1).
The EDS mapping analyses of the B. pumilus before and after Yb(III) adsorption were carried out to reveal the distributions of elements (e.g., C, N, O, P, and Yb) in the cells. Oriented samples were embedded in epoxy resin and dried at 100°C for 3 h. Then, ultrathin sections with a thickness of ca. 75 nm were sliced with a diamond knife using a Lecia EM UC7 ultramicrotome. The sections were placed on carbon-coated copper microgrids for TEM and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) with an FEI Talos F200S microscope at an accelerating voltage of 200 kV.
The change of surface functional groups on B. pumilus before and after Yb(III) was analyzed using a Fourier-transform infrared (FTIR) spectrometer (Vertex 70, Bruker, Germany). Sixty-four scans were collected for each measurement in the spectral range of 4,000 to 400 cm−1 with a resolution of 4 cm−1.
The EDS mapping analyses of the B. pumilus before and after Yb(III) adsorption were carried out to reveal the distributions of elements (e.g., C, N, O, P, and Yb) in the cells. Oriented samples were embedded in epoxy resin and dried at 100°C for 3 h. Then, ultrathin sections with a thickness of ca. 75 nm were sliced with a diamond knife using a Lecia EM UC7 ultramicrotome. The sections were placed on carbon-coated copper microgrids for TEM and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) with an FEI Talos F200S microscope at an accelerating voltage of 200 kV.
The change of surface functional groups on B. pumilus before and after Yb(III) was analyzed using a Fourier-transform infrared (FTIR) spectrometer (Vertex 70, Bruker, Germany). Sixty-four scans were collected for each measurement in the spectral range of 4,000 to 400 cm−1 with a resolution of 4 cm−1.
6
Characterization of Gd2O3@MSN Nanoparticles
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Gd2O3@MSN (1 mg/ml) particle size and zeta potential was confirmed by dynamic light scattering (Malvern Zetasizer Nano ZS system, Malvern, Worcestershire, United Kingdom). We then performed transmission electron microscopy (TEM) (FEI Talos F200S, United States) to examine the surface morphology of the Gd2O3@MSN particles. Their composition was evaluated by energy dispersive X-ray spectroscopy (EDS). Scanning transmission electron microscopy high-angle annular dark-field (STEM-HAADF) images and energy-dispersive X-ray (EDX) element mapping images were obtained using an FEI Talos F200S microscope at an accelerating voltage of 300 kV. The analysis of the N2-adsorption isotherms was performed using Barrett–Joyner–Halenda (BJH) analysis (Micromeritics ASAP-2460, Norcross, GA, United States). The surface area, total pore volume, and average pore distribution curves for the MSNs were determined using the Brunauer–Emmett–Teller (BET) method. Fourier transform infrared refraction (FT-IR, RF-5301PC, Shimadzu, Japan) analyses of the Gd2O3@MSN particles were performed in the range of 400–4,000 cm−1 or structural characterization.
7
Characterization of Insulin-Loaded Nanoparticles
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The sizes and zeta‐potential of the particles were measured by a Zetasizer Nano ZS instrument (Malvern, UK). The morphologies of the particles were detected by a transmission electron microscope (FEI Talos F200S microscope, USA). The circular dichromatic curves of the original Ins before encapsulation into HmA and release of Ins from HmA were recorded by a circular dichromatic spectrometer (Chirascan Plus, UK), and their secondary structures were analyzed by accessary software. A microplate reader (Varioskan LUX Multimode; Thermo, USA) was used to quantitatively analyze the absorbance of CCK‐8‐containing medium. Fluorescence spectra of F‐Ins were monitored by a full wavelength microplate reader (Thermo Fisher, USA). UPLC (Waters, USA) was used to test the concentration of proteins. Imaging of F‐Ins in vivo was performed with a Multimode optical in vivo imaging system (MOIS) (PerkinElmer, USA). Murine routine was examined using animal blood cell analyzer (Mindray, BC‐5000 vet, China). AST and BUN were detected by Fully automatic dry biochemical analyzer (IDEXX, Catalyst One, USA). HE stained sections of organs were imaged by fluorescence microscopy (DMi8 Leica, Germany).
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