Mcherry sec61β

The expression vectors for BCR-ABL, Pickles 2.34, were described previously (Mizutani et al., 2010; Horiguchi et al., 2017) . cDNA for BCR-ABL truncation mutants was generated by PCR with the following primers: BCR_F and BCR_fr1_R, BCR_fr2_F and BCR_fr2_R, BCR_fr3_F and BCR_fr3_R, BCR_fr4_F and BCR_fr4_R, BCR_fr5_F and BCR_fr5_R, BCR-ABL-J_F and BCR-ABL-J_R, ABL_fr1_F and ABL_fr1_R, ABL_fr2_F and ABL_fr2_R, ABL_fr3_F and ABL_fr3_R, ABL_fr4_F and ABL_fr4_R, and ABL_fr5_F and ABL_R. cDNA for BCR-ABL-ΔN was also obtained by PCR with BCR_fr3_F and ABL_R. The resulting PCR products were cleaved by XhoI and NotI and were then subcloned into the XhoI/NotI sites of the pFX-H2B-Venus or the pFX-Venus-Rab5 (Kashiwagi et al., submitted for publication).
cDNAs for FYVE, CD63, LAMP1, and TMEM192 were amplified by PCR with the following primers: FYVE_F and FYVE_R, CD63_F and CD63_R, LAMP1_F and LAMP1_R and TMEM192_F and TMEM192_R. These sequences were then subcloned into the XhoI/NotI sites of the pFX-mCherry vector or the pFX-TFP650 vector (Kashiwagi et al., submitted for publication). mCherry-Sec61 β (# 49155) was obtained from Dr. Gia Voeltz via Addgene (Watertown, MA, USA). Expression vectors for other organelle markers will be described elsewhere (Kashiwagi et al., submitted for publication). The primers used in this study are listed in Table S1.