Genomic DNA samples were submitted to Mayo Clinic’s Advanced Genomics Technology Center for WES. The Bravo liquid handler and Aligent’s protocol was used to prepare paired‐end libraries, and DNA was fragmented using a Covaris E210 sonicator. Agencourt AMPure SPRI beads were used to purify the constructs. SureSelect forward and Agilent SureSelect ILM Pre‐Capture Indexing reverse primers were used to enrich the DNA fragment libraries, which were analyzed with Agilent Bioanalyzer DNA 1000 chip.
Exome capture was performed with the SureSelect XT Human All Exon V5 plus UTR Target Enrichment System (Agilent, Santa Clara, CA). Dynal Dynabeads MyOne Streptavidin T1 captured the DNA:RNA hybrids, and Agencourt Ampure XZP beads eluted DNA from the beads, which were amplified with Agilent Sure Select Post‐Capture Indexing forward and Index PCR reverse primers. Sequencing of the exome libraries was completed with Illumina HiSeq 2000 platform (San Diego, CA) and TruSeq SBS sequencing kit V3 reagents.
Exome capture was performed with the SureSelect XT Human All Exon V5 plus UTR Target Enrichment System (Agilent, Santa Clara, CA). Dynal Dynabeads MyOne Streptavidin T1 captured the DNA:RNA hybrids, and Agencourt Ampure XZP beads eluted DNA from the beads, which were amplified with Agilent Sure Select Post‐Capture Indexing forward and Index PCR reverse primers. Sequencing of the exome libraries was completed with Illumina HiSeq 2000 platform (San Diego, CA) and TruSeq SBS sequencing kit V3 reagents.