N n diisopropylcarbodiimide dic

TentaGel XV RAM resin was obtained from Rapp Polymer (Tuebingen, Germany). Fmoc-protected amino acids, 5(6)-carboxyfluorescein (5,6-FAM) and coupling reagents, 1-hydroxybenzotriazole (HOBt), 2-(6-chloro-1H-benzotriazol-1-yl)-1,1,3,3-tetramethylaminium hexafluorophosphate (HCTU), and benzotriazole-1-yloxytripyrrolidinophosphonium hexafluorophosphate (PYBOP), for peptide synthesis were purchased from Chem-Impex (Wood Dale, IL). N, N’-Diisopropylethylamine (DIPEA) and N, N’-Diisopropylcarbodiimide (DIC) were purchased from Acros Organics (Thermo Fisher Scientific, Waltham, MA). Fmoc-NH-(PEG)₂-COOH (20 atoms) was purchased from EMD Millipore (Billerica, MA). Trifluoroacetic acid (TFA), thioanisole, and HPLC grade solvents (DCM, DMF, acetonitrile, and water) were purchased from Fisher Scientific (Atlanta, GA) or Sigma-Aldrich (St. Louis, MO). Asialofetuin (ASF) and iodine were purchased from Sigma-Aldrich (St. Louis, MO). Fucoidan was purchased from Toronto Research Chemicals (Ontario, Canada). Fluorescein-labelled lectins (AAL and SBA), SBA-Alexa Fluor (488 conjugate), and BSA-conjugated monosaccharides (L-fucose, D-galactose, and N-acetyl-D-galactosamine) were purchased from Vector Labs (Burlingame, CA). The monosaccharides were modified by vinyl sulfone at the anomeric carbon and subsequently conjugated to BSA.^{[51 ,52 ]}