Oligonucleotides of these primers

Manufactured by Thermo Fisher Scientific

Oligonucleotides are synthetic, short DNA or RNA molecules that are commonly used as primers in various molecular biology techniques, such as polymerase chain reaction (PCR) and DNA sequencing. These primers serve as the starting point for DNA synthesis, allowing researchers to amplify or analyze specific regions of genetic material.

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Lab products found in correlation

Random primer, by Thermo Fisher Scientific (2 mentions) Superscript 3 first strand system, by Thermo Fisher Scientific (2 mentions) Power sybr green master mix, by Thermo Fisher Scientific (2 mentions) Rneasy mini kit, by Qiagen (1 mentions)

2 protocols using oligonucleotides of these primers

Zebrafish Liver RNA Extraction and qPCR

Cited 1 time

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For liver tissues of adult zebrafish, total RNA of each sample was purified as described previously [27 (link)]. For cultured cells, total RNA was also purified using the RNeasy Mini Kit. First-strand cDNA was prepared with 200 ng total RNA using the Super Script III First-strand System (Life Technologies) with random primers (Life Technologies). qPCR was performed with Power SYBR Green Master Mix (Applied Biosystems) in triplicate, according to the manufacturer’s protocol. The sequences of the primers are shown in Additional file 3: Table S3. The oligonucleotides of these primers were synthesized by Life Technologies.

Shimada Y., Kuninaga S., Ariyoshi M., Zhang B., Shiina Y., Takahashi Y., Umemoto N., Nishimura Y., Enari H, & Tanaka T. (2015). E2F8 promotes hepatic steatosis through FABP3 expression in diet-induced obesity in zebrafish. Nutrition & Metabolism, 12, 17.

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RNA Extraction and qRT-PCR Analysis in Zebrafish

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For liver tissues of adult zebrafish, total RNA of each sample was purified as described above. For young zebrafish, eight fish were homogenized in RLT buffer using the Mixer Mill 300. Total RNA was purified using the RNeasy Mini Kit (Qiagen), according to the manufacturer's protocol. For cultured cells, total RNA was also purified using the RNeasy Mini Kit. First-strand cDNA was prepared with 200 ng total RNA using the Super Script III First-strand System (Life Technologies, Gaithersburg, MD, USA) with random primers (Life Technologies). qRT-PCR was performed with Power SYBR Green Master Mix (Applied Biosystems) in triplicate, according to the manufacturer's protocol. The sequences of the primers are shown in Supplementary Table S3. The oligonucleotides of these primers were synthesized by Life Technologies.

Hiramitsu M., Shimada Y., Kuroyanagi J., Inoue T., Katagiri T., Zang L., Nishimura Y., Nishimura N, & Tanaka T. (2014). Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis. Scientific Reports, 4, 3708.

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