Male cd 1 mice

Knock-in transgenesis was performed in a vasa-Cas9 expressing An. coluzzii strain which had been introgressed into the Ngousso genetic background [22 (link)]. As a positive control of GFP expression in the midgut we used the An. stephensi line G12-GFP [23 (link)]. Male CD-1 mice (purchased from Janvier Labs and further self-bred at the in-house facility) were used to perform P. berghei infections and feMale CD-1 mice were used for routine blood feedings to propagate mosquito colonies.

Male CD1 mice (n = 15) from Janvier Labs were included in this study at 18 days of age. The animals were maintained in temperature and humidity-controlled facilities. Ambient sound pressure levels inside the cages were below 40 dB SPL. For all hearing test experiments, mice were anesthetized with ketamine (100 mg/kg, i.p.) and xylazine (20 mg/kg, i.p.). Body temperature was maintained at 37°C (Microprobe Thermometer, BAT-12, WPI) with an isothermal pad (Homeothermic Blanket System, Harvard Apparatus). Prior to testing and to exclude middle ear damage, an otoscopic examination (using a binocular operating microscope) was performed on each mouse. Cochlear function was assessed via ABR and DPOAE at postnatal days 18, 21, 25, and 30 (i.e., P18, P21, P25, and P30). All the auditory tests were performed in a sound attenuated and electrically shielded recording chamber. After the final auditory test, animals were sacrificed for histological processing and scanning electron microscopy analyses of hair cell stereocilia. All procedures were approved by the Regional Ethics Committee for animal experiments in France (Comité d'Éthique pour l'Expérimentation Animale Auvergne; EC 92-12).

Male C57BL/6 J mice (n = 88, Janvier) arrived at 8 weeks of age in our animal facility (temperature = 22 ± 2 °C, relative humidity = 50 ± 5%). Upon arrival, mice were single-housed with food/water ad libitum in a light–dark cycle with lights on between 07:00 and 19:00. Animals were habituated to their new housing conditions for 1 week prior to experiments and belonged to one of five cohorts (Table 1). Male CD-1 mice (Janvier) at least 12 weeks of age upon arrival were retired breeders and used as aggressors in the CSD paradigm. Before experiments commenced, the CD-1 mice were tested for sufficient aggressive behavior.