Eif5a

Manufactured by Abcam

Sourced in United States

EIF5A is a protein that plays a role in the regulation of protein synthesis and cell growth. It is involved in the translation elongation process by promoting the formation of the first peptide bond.

Automatically generated - may contain errors

Lab products found in correlation

Eif4a3, by Abcam (1 mentions) Eif4a1, by Abcam (1 mentions) Streptavidin hrp, by Cell Signaling Technology (1 mentions) Eif3a, by Abcam (1 mentions) Eif2α, by Thermo Fisher Scientific (1 mentions) Kdm5a, by Abcam (1 mentions) Eif4e, by Cell Signaling Technology (1 mentions) Eif4g1, by Abcam (1 mentions) Histone h3, by Abcam (1 mentions) Bamh 1 and not 1, by New England Biolabs (1 mentions) Capza1, by Abcam (1 mentions) Pcdna3.1 neo, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

2 protocols using eif5a

Antibody-based Protein Interaction Analysis

Cited 2 times

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The antibodies used were LDH (Santa Cruz, sc-133123), Histone H3 (Abcam, ab1791) Actinin (Santa Cruz, sc-17829), Streptavidin-HRP (Cell Signaling 3999S), eIF3A (Abcam, ab118357), eIF4A1 (Abcam, ab31217), FXR1 (Abcam, ab129089), PABP (Abcam, ab21060), eIF5A (Abcam, ab32443), eIF2α (Invitrogen, AH00802), eIF4G1 (Abcam, ab2609), P53 (Santa-Cruz, sc-126 X), eIF4A3 (Abcam, ab32485), eIF4E (Cell Signaling, 20675), RPS6 (Abcam, ab58350), eIF3D (Abcam, ab12442), S6K1 (Abcam, ab32529), S6K1pT389 (Abcam, ab2571), RPS6pS235 (Abcam, ab12864), KDM5A (Abcam, ab70892). KDM4A and β-Actin antibodies were described in (23 (link)). MCM7 antibody was described in (8 (link)). KDM4A immunoprecipitations were performed with KDM4A-P006.

Van Rechem C., Black J.C., Boukhali M., Aryee M.J., Gräslund S., Haas W., Benes C.H, & Whetstine J.R. (2015). Lysine Demethylase KDM4A Associates with Translation Machinery and Regulates Protein Synthesis. Cancer discovery, 5(3), 255-263.

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Influenza Virus Infection in A549 Cells

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A549 cell line, H7N9 and H1N1pdm09 influenza virus were obtained from Shenzhen Center for Disease Control and Prevention (SZCDC). All the reagents for DIGE were purchased from GE Healthcare (Pittsburgh, PA, USA). DL-Dithiothreitol (DTT), Iodoacetamide (IAA), CHAPS were purchased from Sigma–Aldrich (Louis, MO, USA). The antibodies against CAPZA1, OAT, PCBP1, EIF5A, PAFAH1B2 were purchased from Abcam (Cambridge, MA, USA). The antibody against β-action was purchased from Santa Cruz (Santa Cruz, CA, USA). The secondary antibodies of goat anti-mouse and goat anti-rabbit were purchased from Thermo Fisher (Rockford, IL, USA). The restriction enzyme (BamH I and Not I) were purchased from NEB(USA).
Lipofectamine2000 and pcDNA3.1/Neo(+) were purchased from Invitrogen (Carlsbad, CA, USA).

Ding X., Lu J., Yu R., Wang X., Wang T., Dong F., Peng B., Wu W., Liu H., Geng Y., Zhang R., Ma H., Cheng J., Yu M, & Fang S. (2016). Preliminary Proteomic Analysis of A549 Cells Infected with Avian Influenza Virus H7N9 and Influenza A Virus H1N1. PLoS ONE, 11(5), e0156017.

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