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S 2302

Manufactured by Diapharma
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Sourced in United States

The S-2302 is a laboratory equipment with the core function of measuring and analyzing various samples. It is designed for precise and reliable performance in a wide range of scientific applications.

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Lab products found in correlation

S 2366, by Diapharma (5 mentions) Kallikrein, by Enzyme Research (2 mentions) S 2765, by Diapharma (2 mentions) Kallikrein, by Diapharma (2 mentions) S 2238, by Diapharma (2 mentions) Argatroban, by GlaxoSmithKline (1 mentions) Phosphatidylcholine phosphatidylserine pc ps vesicles, by Avanti Polar Lipids (1 mentions) Z gly gly arg amc, by Bachem (1 mentions) Restriction endonuclease, by Thermo Fisher Scientific (1 mentions) Glutathione agarose, by Thermo Fisher Scientific (1 mentions) Sta ptta reagent, by Diagnostica Stago (1 mentions) Owren koller buffer, by Diagnostica Stago (1 mentions) Prescission protease, by GE Healthcare (1 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Nitrate nitrite fluorometric assay kit, by Cayman Chemical (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Hpaec, by Cambrex (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Ethidium bromide, by Merck Group (1 mentions) Cellular senescence assay kit, by Merck Group (1 mentions)

9 protocols using s 2302

1

Activated Factor XII Coagulation Assay

Cited 2 times
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Pooled normal plasma (PNP), Precision Biologic. FXII-deficient plasma (FXII-dp), George King. FXII, PK, α-kallikrein high molecular weight kinnogen (HK) and corn trypsin inhibitor (CTI), Enzyme Research Lab. PTT-A, Diagnostica Stago. S-2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroaniline), DiaPharma. Size-fractionated Poly-P (60–200 phosphate groups, not calcium saturated) was a gift from Dr. Thomas Renné (Karolinska Institute). Poly-P activity is completely destroyed by acid hydrolysis (8). Antibodies to FXI (O1A6) [9 (link)], FXIIa (559C-X181-D06 [D06]) [10 (link)], and kallikrein (559A-M202-H03 [H03]) are described [10 (link),11 (link)]. Goat polyclonal IgG against human FXII, Affinity Biologicals.
Mohammed B.M., Ivanov I., Matafonov A., Emsley J, & Gailani D. (2017). Activity of factor XII‐Locarno. Research and Practice in Thrombosis and Haemostasis, 2(1), 168-173.
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2

Coagulation Factors and Inhibitors Protocol

Cited 1 time
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Human plasma; Precision Biologicals. α-Thrombin, fXI and fXIa; Hematologic Technologies. Corn trypsin inhibitor (CTI), PK, fXIIa, HK, and HKa; Enzyme Research Lab. RecombiPlasTin human tissue factor; Instrumentation Laboratory. Diisopropylfluorophosphate (DIP), bovine serum albumin (BSA), bovine pancrease deoxyribonuclease I (DNase), and ribonuclease I-AS (RNase); Sigma-Aldrich. Phosphatidylcholine:phosphatidylserine (PC/PS) vesicles, Avanti Polar Lipids. Argatroban, GlaxoSmith Kline. S-2302 (H-D-prolyl-L-phenyl-alanyl-L-arginine-p-nitroaniline dihydrochloride), S-2366 (L-pyro-Glu-L-Pro-L-Arg-p- nitroaniline dihydrochloride); DiaPharma. Z-Gly-Gly-Arg-AMC, Bachem. Genomic DNA was prepared from human blood leukocytes by phenol-chloroform extraction. 32 and 64 base pair species of double stranded DNA were prepared using oligonucleotides generated in the Vanderbilt University Medical Center DNA core facility. RNA was isolated from C57Bl/6 mouse liver in Trizol. DNA and RNA were dissolved in water or TBE buffer, respectively, and stored at -80 0C. Anti-fXI IgG O1A6 (34 (link)) and anti-FXIIa IgG D06 (35 (link)) have been described.
Ivanov I., Shakhawat R., Sun M.F., Dickeson S.K., Puy C., McCarty O.J., Gruber A., Matafonov A, & Gailani D. (2017). Nucleic Acids as Cofactors for Factor XI and Prekallikrein Activation: Different roles for high molecular weight kininogen. Thrombosis and haemostasis, 117(4), 671-681.
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3

Purification of Coagulation Proteases

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The following coagulation and coagulation-related proteases were purchased from Enzyme Research Labs (USA): kallikrein and thrombin [also called factor (F) IIa, FXa, FXIa, and FXIIa]. The following chromogenic substrates were purchased from Diapharma (USA): for thrombin, S-2238; for FXa, S-2765; for FXIa and APC, S-2366; and for kallikrein and FXIIa, S-2302. Custom synthetic double-stranded DNA fragments (gBlocks) were bought from Integrated DNA Technologies (Canada). Restriction endonucleases and glutathione agarose were purchased from Thermo Fisher Scientific (Canada). Nickel chelate affinity resin Ni-NTA agarose was bought from Qiagen (Canada). PreScission Protease [a glutathione sulfotransferase (GST)–human rhinovirus (HRV) 3C protease fusion protein] was purchased from GE Healthcare (Canada). Normal human pooled plasma (NHPP) was produced in-house. FXI-deficient plasma was purchased from Affinity Biologicals (Canada). STA PTTA reagent, STA Neoplastine CI Plus reagent, and Owren-Koller buffer were bought from Diagnostica Stago (Canada).
Hamada M., Bhakta V., Andres S.N, & Sheffield W.P. (2021). Stepwise Reversion of Multiply Mutated Recombinant Antitrypsin Reveals a Selective Inhibitor of Coagulation Factor XIa as Active as the M358R Variant. Frontiers in Cardiovascular Medicine, 8, 647405.
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4

Protein Expression and Senescence Assay

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The HPAECs, EGM, FBS growth supplement, HEPES, 0.25% Trypsin-EDTA, and trypsin-neutralizing solution were used as supplied by Clonetics (San Diego, CA, USA). The HK and PK were purchased from Enzyme Research Laboratory (South Bend, IN, USA). S2302 was purchased from DiaPharma (Franklin, OH, USA). The primary antibody, Goat Anti-Human PRCP, and the secondary Anti-Goat IgG: Whole Molecule, Peroxidase Conjugate were purchased from Bioscience (Long Beach, CA, USA). Mouse Anti-Human β-Actin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The “Nitrate/Nitrite Fluorometric Assay Kit” was purchased from Cayman Chemicals (Ann Arbor, MI, USA). The Cellular Senescence Assay Kit was purchased from Chemicon International (Temecula, CA, USA). TRIzol, Super Signal West Femto Maximum Sensitivity Substrate, RiPA Buffer, Super Signal West Femto Maximum Sensitivity Substrate were supplied by ThermoFisher Scientific (Rockford, IL, USA). The Precision Plus Protein standard (Dual Color) was purchased from Bio-Rad (Rockford, IL, USA). Ethidium bromide was from Sigma-Aldrich (St. Louis, MO, USA).
Boullard N.G., Paris J.J., Shariat-Madar Z, & Mahdi F. (2024). Increased Prolylcarboxypeptidase Expression Can Serve as a Biomarker of Senescence in Culture. Molecules, 29(10), 2219.
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5

Intrinsic Coagulation Pathway Activation by rLundep

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The effect of rLundep on the intrinsic coagulation pathway was based on the generation of human factor XIIa by DNA or aPTT reagent. Ten µl of rLundep (100 nM) or TBS was preincubated at 37°C with 100 µg of salmon sperm DNA (Sigma) or 10 µl of aPTT reagent (Helena Laboratories, Beaumont, TX) in the presence of 5 µl of the chromogenic substrate S2302 (Diapharma, West Chester, OH). TBS alone was utilized as the reaction blank. After 20 minutes, the reaction was initiated by adding 50 µl of citrated-human normal reference plasma (Diagnostica Stago, Inc. Parsippany, NJ) and the amidolytic activity of fXIIa measured at 405 nm in a plate reader (Molecular Devices). All reactions were supplemented with 5 mM MgCl2. Final concentrations of rLundep and S2302 in the assay reaction were 13 nM and 300 µM, respectively.
Chagas A.C., Oliveira F., Debrabant A., Valenzuela J.G., Ribeiro J.M, & Calvo E. (2014). Lundep, a Sand Fly Salivary Endonuclease Increases Leishmania Parasite Survival in Neutrophils and Inhibits XIIa Contact Activation in Human Plasma. PLoS Pathogens, 10(2), e1003923.
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6

Characterization of Thrombin Binding Interactions

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Enzymes were purchased commercially from Haemtech Biopharma Services, except for those used in the experiments shown in section “Binding of dansyl Compound 2 to thrombin” below, where wild-type thrombin and mutant S195A were expressed as prethrombin-2 in E. coli, refolded, and purified to homogeneity as previously described [17 ]. Chromogenic substrates S-2238, S-2302, S-2366, and S-2765 were purchased from Diapharma, Spectrozyme was purchased from Sekisui Diagnostics, and fibrinogen was purchased from Sigma.
Sivaraja M., Pozzi N., Rienzo M., Lin K., Shiau T.P., Clemens D.M., Igoudin L., Zalicki P., Chang S.S., Estiarte M.A., Short K.M., Williams D.C., Datta A., Di Cera E, & Kita D.B. (2018). Reversible covalent direct thrombin inhibitors. PLoS ONE, 13(8), e0201377.
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7

Kinetic Assay and ELISA for C1-INH Activity

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PdC1-INH (Cetor®) was obtained from the Sanquin Blood Supply Foundation, The Netherlands. Monoclonal antibody (mAb) RII and polyclonal antibodies against C1-INH were produced previously [36 (link),37 (link)]. C1s for kinetic assays and ELISA was purchased from Calbiochem (La Jolla, CA, USA). C1s and pAb against C1-INH were biotinylated using EZ-Link® Sulfo-NHS-Biotin (Thermo Fisher Scientific, Waltham, MA, USA) or Pierce (Rockford, ILL) following the manufacturer protocol. Human Factor XIIa and kallikrein were purchased from Kordia (Leiden, The Netherlands). Agarose-bound RCA120 and biotinylated-RCA120 were from Vector Labs, USA. Streptavidin-peroxidase was obtained from Amersham Pharmacia Biotech (Uppsala, Sweden). Streptavidin-polymerized peroxidase (poly-HRP) was obtained from Sanquin (Business Unit Reagents, Amsterdam, The Netherlands). Chromogenic substrates S2251, S-2302 and S2366 were from DiaPharma (Beckett Ridge, Ohio, USA); C1s substrate H-D-Val-Ser-Arg.p-NA.2HCl was custom synthesized by Biomatik (Wilmington, DE, USA).
Zeerleder S., Engel R., Zhang T., Roem D., van Mierlo G., Wagenaar-Bos I., van Ham S.M., Wuhrer M., Wouters D, & Jongerius I. (2021). Sugar Matters: Improving In Vivo Clearance Rate of Highly Glycosylated Recombinant Plasma Proteins for Therapeutic Use. Pharmaceuticals, 14(1), 54.
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8

Purified Coagulation Protease Assays

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Purified human coagulation proteases FXIa, thrombin, FXa, FXIIa, kallikrein, and APC were from Enzyme Research Labs (South Bend, IN, USA). Chromogenic substrates were purchased from Diapharma (West Chester, OH, USA): for FXIa and APC, S-2366; for kallikrein and FXIIa, S-2302; for thrombin, S-2238; and for FXa, S-2765.
Bhakta V., Hamada M., Nouanesengsy A., Lapierre J., Perruzza D.L, & Sheffield W.P. (2021). Identification of an alpha-1 antitrypsin variant with enhanced specificity for factor XIa by phage display, bacterial expression, and combinatorial mutagenesis. Scientific Reports, 11, 5565.
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9

Quantifying Factor XIIa Activity

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Factor XIIa (20 nM) final was added to HBS/0.1% PEG/2 mM CaCl 2 /10.8 lM ZnCl 2 (pH 7.4 or pH 7.0) at 37 °C. After the addition of CTI (24 nM final), aliquots were removed at predetermined time intervals and added to 500 lM S-2302 (DiaPharma, West Chester, OH, USA). The reaction was diluted only 5% by the chromogenic substrate. Estimations of the free FXIIa concentration present at equilibrium were calculated as for TFPI.
Gissel M., Brummel-Ziedins K.E., Butenas S., Pusateri A.E., Mann K.G, & Orfeo T. (2016). Effects of an acidic environment on coagulation dynamics. Journal of thrombosis and haemostasis : JTH, 14(10).
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