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Aspectramax m5

Manufactured by Molecular Devices
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The AspectraMax M5 is a multi-mode microplate reader that can perform a variety of spectroscopic measurements, including absorbance, fluorescence, and luminescence. It is designed to provide accurate and reliable data for a wide range of applications in life science research and drug discovery.

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Lab products found in correlation

Amplexultrared soluble fluorophore, by Thermo Fisher Scientific (2 mentions) Rplpo antibody, by Proteintech (2 mentions)

2 protocols using aspectramax m5

1

Direct Binding Duplex ELISA Immunoassay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Direct binding duplex ELISAs measured immunoreactivity with results
normalized to large acidic ribosomal protein (RPLPO) [33 (link)]. Immunoreactivity to target proteins was
detected with horseradish peroxidase-conjugated secondary antibody and Amplex
UltraRed soluble fluorophore (Invitrogen, Carlsbad, CA). RPLPO antibody
(Proteintech Group Inc, Chicago, IL) was biotinylated, and its immunoreactivity
was detected with streptavidin-conjugated alkaline phosphatase and the
4-Methylumbelliferyl phosphate (4-MUP) substrate. Fluorescence intensities
(Amplex Red: Ex 565 nm/Em 595 nm; 4-MUP: Ex360/Em450) was measured in a
SpectraMax M5 (Molecular Devices, Sunnyvale, CA). Antibody omission controls
were included. The calculated target protein/RPLPO ratios were used for
inter-group comparisons.
Andreani T., Tong M., Gundogan F., Silbermann E, & de la Monte S.M. (2016). Differential Effects of 3rd Trimester-Equivalent Binge Ethanol and Tobacco-Specific Nitrosamine Ketone Exposures on Brain Insulin Signaling in Adolescence. Journal of diabetes and related disorders, 1(1), 105.
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2

Direct Binding Duplex ELISA Immunoassay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Direct binding duplex ELISAs measured immunoreactivity with results
normalized to large acidic ribosomal protein (RPLPO) [33 (link)]. Immunoreactivity to target proteins was
detected with horseradish peroxidase-conjugated secondary antibody and Amplex
UltraRed soluble fluorophore (Invitrogen, Carlsbad, CA). RPLPO antibody
(Proteintech Group Inc, Chicago, IL) was biotinylated, and its immunoreactivity
was detected with streptavidin-conjugated alkaline phosphatase and the
4-Methylumbelliferyl phosphate (4-MUP) substrate. Fluorescence intensities
(Amplex Red: Ex 565 nm/Em 595 nm; 4-MUP: Ex360/Em450) was measured in a
SpectraMax M5 (Molecular Devices, Sunnyvale, CA). Antibody omission controls
were included. The calculated target protein/RPLPO ratios were used for
inter-group comparisons.
Andreani T., Tong M., Gundogan F., Silbermann E, & de la Monte S.M. (2016). Differential Effects of 3rd Trimester-Equivalent Binge Ethanol and Tobacco-Specific Nitrosamine Ketone Exposures on Brain Insulin Signaling in Adolescence. Journal of diabetes and related disorders, 1(1), 105.
+ Open protocol
+ Expand

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