U251 and U87 human glioma cells were transfected at 80% confluence with SCAP siRNA (cat. no. sc-36462; Santa Cruz Biotechnology, Inc.), hypoxia-inducible factor 1 (HIF-1) siRNA (cat. no. sc-35561; Santa Cruz Biotechnology, Inc.), SREBP-1 siRNA (cat. no. sc-36557; Santa Cruz Biotechnology, Inc.) and control siRNA (cat. no. sc-37007; Santa Cruz Biotechnology, Inc.) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. Briefly, prior to treatment, Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.)/siRNA complexes were prepared in Opti-MEM medium, at the ratio of 1 μl Lipofectamine 2000 per 20 pmole siRNA. Cells then were then treated at 37°C for 48 h before being replaced with complete medium for the desired duration. The transfection efficiency of the cells was verified by western blotting.
Srebp1 sirna
Manufactured by Santa Cruz Biotechnology
SREBP1 siRNA is a synthetic small interfering RNA (siRNA) designed to target and silence the expression of the SREBP1 (Sterol Regulatory Element-Binding Protein 1) gene. SREBP1 is a transcription factor involved in the regulation of lipid metabolism. This product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Hif 1 sirna, by Santa Cruz Biotechnology (1 mentions)
Control sirna, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
3 protocols using srebp1 sirna
1
Silencing Key Regulators in Glioma Cell Lines
2
Silencing FASN and Tubulin Genes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were incubated in Dulbecco’s modified Eagle medium/F-12 culture medium with 10% fetal bovine serum (Gibco, 16,000–044) and 100 U/ml penicillin and streptomycin. All cells were cultured in a humidified incubator containing 5% CO2 at 37 °C.
For knockdown of FASN mRNA, negative control siRNA (5′- UUCUCCGAACGUGUCACGUTT-3′), FASN siRNA (5′-CAGAGUCGGAGAACUUGCAGGAGUU-3′), α-tubulin siRNA (5′-AAAGATGTCAATGCTGCCATT-3′), and β-tubulin siRNA (5′-CCCAGCGGCAACTACGTGGG-3′) were designed [1 (link), 16 (link), 24 (link)], SREBP1 siRNA was purchased from Santa Cruz Biotech (sc-36558). Cells were plated in six-well plates at a density of 1 × 106/well and cultured for 24 h. Then the cells were transfected with the FASN and α-tubulin siRNA, respectively (30 pmol/well) by LipofectamineTM RNAiMAX (Invitrogen, 13778075) when cells reach ~80% confluency.
For knockdown of FASN mRNA, negative control siRNA (5′- UUCUCCGAACGUGUCACGUTT-3′), FASN siRNA (5′-CAGAGUCGGAGAACUUGCAGGAGUU-3′), α-tubulin siRNA (5′-AAAGATGTCAATGCTGCCATT-3′), and β-tubulin siRNA (5′-CCCAGCGGCAACTACGTGGG-3′) were designed [1 (link), 16 (link), 24 (link)], SREBP1 siRNA was purchased from Santa Cruz Biotech (sc-36558). Cells were plated in six-well plates at a density of 1 × 106/well and cultured for 24 h. Then the cells were transfected with the FASN and α-tubulin siRNA, respectively (30 pmol/well) by LipofectamineTM RNAiMAX (Invitrogen, 13778075) when cells reach ~80% confluency.
3
Modulating SREBP1 and FBXW7 Pathways
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