1200 series lc ms system

Manufactured by Agilent Technologies

The Agilent 1200 series LC/MS system is a liquid chromatography-mass spectrometry (LC/MS) instrument designed for analytical applications. It combines high-performance liquid chromatography (HPLC) with mass spectrometry technology to provide separation, identification, and quantification of chemical compounds in a sample. The system features modular components that can be configured to meet specific analytical requirements.

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Lab products found in correlation

Sephadex lh 20, by GE Healthcare (2 mentions) Pack ods a column, by YMC (2 mentions) Avance 3 400 nmr spectrometer, by Bruker (2 mentions) 6250tof lc ms, by Agilent Technologies (2 mentions) J 815 spectropolarimeter, by Jasco (1 mentions) Silica gel, by Qingdao Marine Chemical (1 mentions) Flexar system, by PerkinElmer (1 mentions) 6120 quadrupolems detector, by Agilent Technologies (1 mentions) Combiflash rf flash chromatography system, by Teledyne (1 mentions) J 810 spectropolarimeter, by Jasco (1 mentions)

Close spelling variants of this product

1200 series (380 citations) 1200 series system (61 citations) 1200 system (59 citations) 1200 LC system (41 citations) 1200 series LC system (37 citations) LC 1200 (35 citations) 1200 Series LC (28 citations) LC-MS system (22 citations) 1200 LC/MS (3 citations)

5 protocols using 1200 series lc ms system

Characterization of Bioactive Compounds

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Ultraviolet (UV) measurements were taken using a NanoDrop 2000 ultra-micro spectrophotometer. CD spectra were recorded using a JASCO J-815 spectropolarimeter. NMR spectra were determined using a Bruker Avance III 400 NMR spectrometer (¹H, 400 MHz, ¹³C, 100 MHz). Low-resolution electrospray ionization mass spectrometry (ESIMS) data were collected on an Agilent1200 series LC/MS system, and high-resolution ESIMS (HRESIMS) data were acquired on an Agilent 6250TOF LC/MS. Semipreparative HPLC was performed on a Calmflowplus system that was equipped with a 50D UV–vis Detector (Lumiere Tech Ltd) and a YMC Pack ODS-A column (10 mm × 250 mm 5 μm, Japan). Silica gel (200–300 mesh, Qingdao Marine Chemical Factory, Qingdao, China), ODS (YMC, Japan), and Sephadex LH-20 (GE Healthcare BioSciences AB, Sweden) were used as packing materials for column chromatography. All chemicals used in the study were of analytical grade.

Guo Q., Shen S., Wang X., Shi L., Ren Y., Li D., Yin Z., Zhang J., Yang B., Wang X., Ding G, & Chen L. (2024). Bioactive cytochalasans from the desert soil-derived fungus Chaetomium madrasense 375 obtained via a chemical engineering strategy. Frontiers in Microbiology, 14, 1292870.

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Time-course Monitoring of Ampicillin Reactions

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The ampicillin sodium salt (150 mg, 0.4 mmol) and the carbonyl compound (8.6 mmol) were dissolved in H₂O (30 mL) and the solution was acidified to pH 2. The solution was then aliquoted into three microwave reaction vessels (10 mL per vessel). Each vessel was then heated in heating block at 100 °C with stirring. Samples were taken and immediately frozen on dry ice after 0 min (before heating) and after 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, 90 minutes, 105 minutes, 2 hours, 2.5 hours, 3 hours, 4 hours, and 5 hours heating. The samples were then analysed by LC/MS using an Agilent Technologies 1200 series LC/MS system with an H₂O/MeCN solvent system (if a precipitate formed during the reaction, the samples were diluted 1:1 with acetonitrile (MeCN) to dissolve the precipitate before analysis). MS signal intensities for each product were extracted from the spectra and were calibrated using purified controls (products 1-5). A control time-course without added carbonyl was also conducted, which revealed formation of 2.

Reinbold R., John T., Spingardi P., Kawamura A., Thompson A.L., Schofield C.J, & Hopkinson R.J. (2019). Formaldehyde quantification using ampicillin is not selective. Scientific Reports, 9, 18289.

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Solid-Phase Peptide Synthesis and Purification

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All peptides were synthesized using standard solid-phase peptide synthesis in an automated microwave-assisted peptide synthesizer (CEM Liberty 1) using Fmoc-protected amino acids. Peptide cleavage was performed using a cleavage cocktail containing 7.6 mL trifluoroacetic acid (TFA), 0.2 mL triisopropylsilane (TIS), 400 mg phenol, and 0.2 mL double distilled water. The peptides were cleaved from the resin for ~3 h at room temperature and precipitated in cold ethyl ether. The cleaved peptides were dried in vacuo and purified by reverse phase HPLC (PerkinElmer Flexar system) using 95%/5% (v/v) water/acetonitrile with trace (0.1vol.%) of TFA as the starting solvent mobile phase. A linear gradient of acetonitrile was used to separate the products through a semi-prep scale peptide C18 column at 5 mL/min flow rate. The separation processes were monitored with a UV/vis detector at 280 nm (for peptides with tyrosine residue) or 220 nm (for peptides without tyrosine residue). Purified peptides were characterized with liquid chromatography coupled with mass spectrometry (Agilent Technologies, 1200 series LC/MS system).

Nguyen H.D., Liu H.Y., Hudson B.N, & Lin C.C. (2019). Enzymatic Cross-Linking of Dynamic Thiol-Norbornene Click Hydrogels. ACS biomaterials science & engineering, 5(3), 1247-1256.

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Purification and Characterization of Organic Compounds

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All materials were obtained from commercial suppliers and used without purification, unless otherwise noted. Dry organic solvents, packaged under nitrogen in septum-sealed bottles, were purchased from EMD Millipore and Sigma-Aldrich Co. Reactions were monitored using EMD silica gel 60 F₂₅₄ TLC plates or using an Agilent 1200 series LCMS system with a diode array detector and an Agilent 6120 quadrupole MS detector. Compound purification was accomplished by liquid chromatography on a Teledyne Isco CombiFlash RF+ flash chromatography system. Nuclear magnetic resonance (NMR) spectra were recorded on an Agilent NMR spectrometer (400 MHz) at room temperature. Chemical shifts are reported in ppm relative to residual solvent signal. The residual shifts were taken as internal references and reported in ppm.

Sourimant J., Lieber C.M., Yoon J.J., Toots M., Govindarajan M., Udumula V., Sakamoto K., Natchus M.G., Patti J., Vernachio J, & Plemper R.K. (2022). Orally efficacious lead of the AVG inhibitor series targeting a dynamic interface in the respiratory syncytial virus polymerase. Science Advances, 8(25), eabo2236.

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Spectroscopic Characterization of Compounds

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CD spectra were determined on a JASCO J-810 spectropolarimeter (JASCO Corporation), and UV data were recorded using a PERSEE UV-VIS spectrophotometer T9 (Beijing, China). NMR experiments were carried out on a Bruker AVANCE III 400 NMR spectrometer (Bruker, Germany), using tetramethylsilane (TMS) or solvent signals as an internal reference. HRESIMS data were collected on an Agilent 6250 TOF LC/MS, and ESIMS data were acquired on an Agilent 1200 series LC/MS system. Semipreparative HPLC was run on a Calmflow^plus system that was equipped with a YMC Pack ODS-A column (10 mm × 250 mm 5 μm, Japan) and a 50D UV-vis Detector (Lumiere Tech Ltd) and with a flow rate of 2.0 ml/min. Packing materials for column chromatography were silica gel (200–300 mesh; Qingdao Marine Chemical Factory, Qingdao, China), ODS (YMC, Japan), and Sephadex LH-20 (GE Healthcare BioSciences AB, Sweden). All chemicals used in the study were of analytical grade.

Guo Q., Chen J., Ren Y., Yin Z., Zhang J., Yang B., Wang X., Yin W., Zhang W., Ding G, & Chen L. (2021). Hydrazine-Containing Heterocycle Cytochalasan Derivatives From Hydrazinolysis of Extracts of a Desert Soil-Derived Fungus Chaetomium madrasense 375. Frontiers in Chemistry, 9, 620589.

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