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Cd279 pd 1

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CD279 (PD-1) is a cell surface receptor that belongs to the immunoglobulin superfamily. It is primarily expressed on activated T cells, B cells, and myeloid cells. CD279 (PD-1) plays a crucial role in the regulation of the immune response by negatively regulating T cell activity, thus preventing autoimmunity and promoting immune tolerance.

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Lab products found in correlation

Kaluza flow analysis software, by Beckman Coulter (2 mentions) Gallios flow cytometer, by Beckman Coulter (2 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Cd62l, by Beckman Coulter (1 mentions)

Spelling variants of this product

CD279 (PD-1) BV421 (3 citations) Anti-CD279 (PD-1) APC (2 citations) PE-CF594 anti-CD279 (PD-1) (2 citations) PerCP-Cy5.5 Mouse Anti-Human CD279 (PD-1) (2 citations)

2 protocols using cd279 pd 1

1

Multiparameter Analysis of T Cell Subsets

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MultiVSTs were surface-stained with monoclonal antibodies to: CD45RO, CD197 (CCR7), CD279 (PD-1) [Becton Dickinson (BD), Franklin Lakes, NJ], CD3, CD4, CD8, CD16, CD56, CD62L (Beckman Coulter, Brea, CA), and CD366 (TIM-3), CD223 (LAG3) (BioLegend, San Diego, CA). Cells (0.5x106 per tube) were pelleted in phosphate-buffered saline (PBS) (Sigma-Aldrich), then antibodies added in saturating amounts (5 μl) followed by incubation for 15 mins at 4°C. Subsequently, cells were washed, resuspended in 300μl of PBS and at least 50,000 live cells acquired on a Gallios™ Flow Cytometer (Beckman Coulter) and analyzed with Kaluza® Flow Analysis Software (Beckman Coulter).
Koukoulias K., Papayanni P.G., Jones J., Kuvalekar M., Watanabe A., Velazquez Y., Gilmore S., Papadopoulou A., Leen A.M, & Vasileiou S. (2023). Assessment of the cytolytic potential of a multivirus-targeted T cell therapy using a vital dye-based, flow cytometric assay. Frontiers in Immunology, 14, 1299512.
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2

Multiparametric Immune Cell Profiling

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Multi-R-VST were surface-stained with monoclonal antibodies to: CD3, CD25, CD28, CD45RO, CD279 (PD-1) [Becton Dickinson (BD), Franklin Lakes, NJ, USA], CD4, CD8, CD16, CD62L, CD69 (Beckman Coulter, Brea, CA, USA) and CD366 (TIM-3) (BioLegend, San Diego, CA, USA). Cells were acquired on a Gallios™ Flow Cytometer and analyzed with Kaluza® Flow Analysis Software (Beckman Coulter). See Online Supplementary Appendix for details.
Vasileiou S., Turney A.M., Kuvalekar M., Mukhi S.S., Watanabe A., Lulla P., Ramos C.A., Naik S., Vera J.F., Tzannou I, & Leen A.M. (2020). Rapid generation of multivirus-specific T lymphocytes for the prevention and treatment of respiratory viral infections. Haematologica, 105(1), 235-243.
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