Rabbit igg anti human mouse c1q

Manufactured by Thermo Fisher Scientific

Rabbit IgG anti-human/mouse C1q is a laboratory reagent that is used to detect and quantify the presence of the C1q protein in human and mouse samples. C1q is a component of the complement system, which is part of the immune response. This product can be used in various immunological assays and research applications.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (4 mentions) Streptavidin hrp, by R&D Systems (2 mentions) Rabbit igg anti factor b, by Thermo Fisher Scientific (2 mentions) Mouse igg anti human igm, by Novus Biologicals (2 mentions) Donkey anti mouse igg hrp, by Thermo Fisher Scientific (2 mentions) Chemi imager, by Azure Biosystems (2 mentions) Mini protean tgx gel, by Bio-Rad (2 mentions) Goat anti rabbit igg hrp, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rabbit anti-human C1q Rabbit anti-human IgG Mouse anti-IgG IgG Rabbit

2 protocols using rabbit igg anti human mouse c1q

Western Blot Analysis of Complement Proteins

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Serum samples were incubated in RIPA buffer (Thermofisher) supplemented with protease inhibitor (Pierce). Total protein was quantified using BSA protein assay kit (Pierce). Proteins were resolved in Mini‐PROTEAN TGX gels (Bio‐Rad) and transferred to polyvinylidene membrane (Immobilion). C1q was detected with rabbit IgG anti‐human/mouse C1q (Invitrogen). Factor B was detected with rabbit IgG anti‐factor B (Invitrogen). Human IgM was detected with mouse IgG anti‐human IgM (Novusbio) with goat anti rabbit‐IgG‐HRP or donkey anti‐mouse‐IgG‐HRP (Invitrogen) as a secondary antibody. Streptavidin‐HRP (R&D systems) and crescendo western HRP substrate (Immobilon) were used prior to acquisition with Chemi‐Imager (Azure Biosystems).

Dahmani M., Cook J.H., Zhu J.C, & Riley S.P. (2021). Contribution of classical complement activation and IgM to the control of Rickettsia infection. Molecular Microbiology, 116(6), 1476-1488.

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Quantification of Complement Proteins

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Serum samples were incubated in RIPA buffer (Thermofisher) supplemented with protease inhibitor (Pierce). Total protein was quantified using BSA protein assay kit (Pierce). Proteins were resolved in Mini-PROTEAN TGX gels (Bio-Rad) and transferred to polyvinylidene membrane (Immobilion). C1q was detected with rabbit IgG anti-human/mouse C1q (Invitrogen). Factor B was detected with rabbit IgG anti-factor B (Invitrogen). Human IgM was detected with mouse IgG anti-human IgM (Novusbio) with goat anti rabbit-IgG-HRP or donkey anti-mouse-IgG-HRP (Invitrogen) as a secondary antibody. Streptavidin-HRP (R&D systems) and crescendo western HRP substrate (Immobilon) were used prior to acquisition with Chemi-Imager (Azure Biosystems).

Dahmani M., Cook J.H., Zhu J.C, & Riley S.P. (2021). Contribution of classical complement activation and IgM to the control of Rickettsia infection. Molecular Microbiology, 116(6), 1476-1488.

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