6 monoacetylmorphine

Manufactured by Cerilliant

Sourced in United States

6-monoacetylmorphine is a chemical standard used for analytical purposes. It is a metabolite of heroin and can be used for identification and quantification in analytical procedures.

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3 protocols using 6 monoacetylmorphine

Apoptosis Induction Assay with Drugs

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Experimental drugs stock solutions were prepared at concentrations of 3 mM in ethanol 70%, obtained from Sigma Aldrich (St. Louis, MO). Stock solutions were kept in sterile glass vials and stored at 4°C. The positive control, a known apoptosis inducer, camptothecin, cocaine, and xylazine were obtained from Sigma Aldrich; the heroin metabolite (6-monoacetylmorphine) was obtained from Cerilliant Corporation (Round Rock, Texas). The reagent sodium hydroxide (NaOH), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), and 2,3-diaminonaphthalene (DAN) were obtained from Sigma Aldrich (St. Louis, MO). Stock solutions of DCFH-DA were prepared at concentrations of 10 mM in dimethyl sulfoxide (DMSO) biotechnology grade, obtained from Sigma Aldrich, St. Louis, MO. Stock solutions were kept in sterile glass vials and stored at 4°C. DNA fragmentation reagent, 4′,6-diamidino-2-phenylindole (DAPI), was obtained from Chemometec (Allerød, Denmark).

Silva-Torres L., Veléz C., Álvarez L, & Zayas B. (2014). Xylazine as a Drug of Abuse and Its Effects on the Generation of Reactive Species and DNA Damage on Human Umbilical Vein Endothelial Cells. Journal of Toxicology, 2014, 492609.

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Certified Reference Standards for Drugs

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Certified reference standards of 6-monoacetylmorphine, alprazolam, amphetamine, buprenorphine, benzoylecgonine, carisoprodol, chlordiazepoxide, clonazepam, cocaine, codeine, cotinine, dihydrocodeine, diazepam, EDDP, fentanyl, flurazepam, hydrocodone, hydromorphone, lorazepam, MDA, MDEA, MDMA, methamphetamine, meperidine, morphine, methylphenidate, methadone, naloxone, norbuprenorphine, nicotine, nordiazepam, norfentanyl, noroxycodone, alpha-hydroxyalprazolam, oxazepam, oxycodone, oxymorphone, tapentadol, temazepam, ll-nor-9-Carboxy-Δ9-THC, and cis-tramadol were purchased, each at a concentration of 1 mg/mL, from Cerilliant Corporation (Round Rock, TX). Isotopically labeled internal standards (IS) morphine-D3, codeine- D6, hydrocodone-D3, 6-monoacetylmorphine-D6, hydromorphone-D6, oxymorphone-D3, noroxycodone-D3, and naloxone-D5 were purchased, each at a concentration of 100 μg/mL, from Cerilliant Corporation (Round Rock, TX). Drug-free urine was obtained from Bio-Rad Laboratories (Irvine, CA). High-performance liquid chromatography (HPLC) grade water, methanol, and formic acid were purchased from Fisher Scientific (Pittsburgh, PA) and ammonium formate was purchased from Sigma Aldrich (St. Louis, MO).

Pablo A., Breaud A.R, & Clarke W. (2019). Automated Analysis of Dried Urine Spot (DUS) Samples for Toxicology Screening. Clinical biochemistry, 75, 70-77.

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Comprehensive Analytical Reference Standards

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Certified reference materials of amitriptyline, bupropion, citalopram, desipramine, desmethylcitalopram, duloxetine, fluoxetine, imipramine, mirtazapine, nortriptyline, paroxetine, sertraline and trazodone were purchased from LGC Standards (Teddington, London, UK); certified reference materials of clomipramine, desmethylvenlafaxine, doxepin, hydroxybupropion, norfluoxetine, norsertraline, trimipramine, venlafaxine, 7-amino clonazepam, 7-amino flu nitrazepam, alprazolam, bromazepam, clonazepam, diazepam, flu nitrazepam, lorazepam, midazolam, nitrazepam, nordiazepam, oxazepam, temazepam, zolpidem, 6-monoacetyl morphine, N-desmethyltramadol, buprenorphine, codeine, fentanyl, hydrocodone, hydromorphone, meperidine, methadone, morphine, naloxone, naltrexone, oxycodone, oxymorphone, tramadol, bupropion-d 9 , citalopram-d 6 , clonazepam-d 4 , codeine-d 3 , diazepam-d 5 , duloxetine-d 3 and morphine-d 3 from Cerilliant (Round Rock, TX, USA); methanol, acetonitrile, ammonium formate and formic acid from Merck (Darmstadt, Germany); ultrapure deionized water was purified by Milli-Q from Millipore (Billerica, MA, USA). All solvents used in the extraction procedure were HPLC grade. Quantisal™ oral fluid collection devices and elution buffer were purchased from Immunalysis (Pomona, CA, USA).

Arantes A.C.F., Cunha K.F.d., Cardoso M.S., Oliveira K.D., & Costa J.L. (2020). Development and validation of quantitative analytical method for 50 drugs of antidepressants, benzodiazepines and opioids in oral fluid samples by liquid chromatography–tandem mass spectrometry. Forensic Toxicology, 39(1), 179-197.

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