Blood was drawn by aseptic venipuncture from an antecubital vein using a 21-gauge butterfly needle (0.8 x 19 mm; Greiner Bio-One, Kremsmünster, Austria) one day after the percutaneous intervention. To avoid procedural deviations all blood samples were taken by the same physician applying a light tourniquet, which was immediately released and the samples were mixed adequately by gently inverting the tubes. After the initial 3ml of blood had been discarded to reduce procedurally-induced platelet activation, blood was drawn into 3.8% sodium citrate Vacuette tubes (Greiner Bio-One; 9 parts of whole blood, 1 part of sodium citrate 0.129 M/L) for evaluations by LTA and flow cytometry, into 3.2% sodium citrate Vacuette tubes (Greiner Bio-One; 9 parts of whole blood, 1 part of sodium citrate 0.109 M/L) for the VerifyNow P2Y12 and aspirin assays, and into Vacuette tubes containing lithium heparin (18 IU/ ml) for the determinations by MEA. To avoid investigator-related variations of the results, each of the different tests was performed by just one corresponding operator, who was blinded to the results from the other operators.
Sodium citrate vacuette tube
Manufactured by Greiner
Sourced in Germany, Austria
The 3.8% sodium citrate vacuette tube is a laboratory specimen collection device used to collect blood samples. It contains a 3.8% sodium citrate solution, which acts as an anticoagulant to prevent the blood from clotting during collection and processing.
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7 protocols using sodium citrate vacuette tube
1
Blood Sampling for Platelet Function Analysis
2
Platelet-Poor Plasma Isolation
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Blood was collected at the St. Antonius Hospital (Nieuwegein, The Netherlands) between April 2010 and August 2012. A time window of at least 3 months was required between the index PCI (controls) or the PCI for stent thrombosis (cases) and blood sampling. Venous blood was collected from the antecubital vein using 21-gauge needles and 3.2% (w/v) sodium citrate Vacuette tubes (Greiner Bio-one, Frickenhausen, Germany). The first 5 mL of free-flowing blood was discarded to avoid haemostatic activation. Platelet-poor plasma (PPP) was obtained by two separate centrifugation steps of 10 min at 150×g, followed by 10 min at 16.000×g. All aliquots were stored at −80°C until analysis.
3
Thrombin Generation Potential Analysis
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Blood was drawn by aseptic venipuncture from an antecubital vein using a 21-gauge butterfly needle (0.8 × 19 mm; Greiner Bio-One, Kremsmünster, Austria) 1 day after the percutaneous intervention. To avoid procedural deviations all blood samples were taken by the same physician applying a light tourniquet, which was immediately released and the samples were mixed adequately by gently inverting the tubes. After the initial 3 ml of blood had been discarded to reduce procedurally-induced platelet activation, blood was drawn into 3.8% sodium citrate Vacuette tubes (Greiner Bio-One; 9 parts of whole blood, 1 part of sodium citrate 0.129 M/l) for the measurement of thrombin generation potential.
4
Blood Sample Preparation Protocol
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Blood was drawn within 24 hours after admission from the arterial or central venous line. After the initial 3 mL of blood had been discarded, blood was drawn into a serum separator tube and centrifuged at 4°C at 3000 RPM for 15 minutes and stored at -80°C for later analysis. In addition, an EDTA-tube and a 3.8% sodium citrate vacuette tube (all Greiner Bio-One) were collected and treated as described above for later analysis.
5
Blood Collection for Coronary Angiography
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Blood was drawn in the morning prior to elective coronary angiography after venipuncture from an antecubital vein using a 21-gauge butterfly needle (0.8 mm × 19 mm; Greiner Bio-One, Kremsmünster, Austria). After the initial 3 mL of blood were discarded, blood was drawn into an EDTA tube (Greiner Bio-One) for immediate analysis by flow cytometry. Furthermore, a 3.8% sodium citrate Vacuette tube (Greiner Bio-One; nine parts of whole blood, one part of sodium citrate 0.129 M/L), a serum separator tube (Greiner Bio-One) and an EDTA tube (Greiner Bio-One) were collected, immediately centrifuged (4°C; 3000RPM for 15 min) and stored at—80°C for later analysis.
6
Blood Sample Collection for Coronary Angiography
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Blood was drawn in the morning prior to elective coronary angiography after venipuncture from an antecubital vein using a 21-gauge butterfly needle (0.8 mm × 19 mm; Greiner Bio-One, Kremsmünster, Austria). After the initial 3 mL of blood were discarded, blood was drawn into an EDTA tube (Greiner Bio-One) for immediate analysis by flow cytometry. Furthermore, a 3.8% sodium citrate Vacuette tube (Greiner Bio-One; nine parts of whole blood, one part of sodium citrate 0.129 M/L), a serum separator tube (Greiner Bio-One) and an EDTA tube (Greiner Bio-One) were collected, immediately centrifuged (4 °C; 3000RPM for 15 min) and stored at −80 °C for later analysis.
7
Blood Sampling Protocol for Biomarker Analysis
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Blood was drawn at the time of admission (24 h time window) from an arterial or central venous line. A serum separator tube, an EDTA‐tube and a 3.8% sodium citrate vacuette tube (Greiner Bio‐One, Austria) were used for collection after discarding the initial 3 mL of blood to ensure stable sampling conditions. Consecutively, samples were centrifuged at 4°C and 3000 RPM for 15 min and stored at −80°C for later analysis. Standard laboratory values including NT‐proBNP were carried out by the department of laboratory medicine of the Vienna general hospital.
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