Log In Sign up
The largest database of trusted experimental protocols

Anti cd4 pe antibody

Manufactured by Southern Biotech
Visit Supplier

The Anti-CD4-PE antibody is a laboratory reagent used for the detection and identification of CD4-positive cells in flow cytometry applications. It binds specifically to the CD4 surface antigen and is conjugated with the fluorescent dye Phycoerythrin (PE) to enable fluorescent labeling and visualization of CD4-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

Facscalibur, by BD (1 mentions) Accuri c6, by BD (1 mentions)

Close spelling variants of this product

CD4-PE Anti-CD4

2 protocols using anti cd4 pe antibody

1

CFSE-based T cell proliferation assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Splenocytes from the immunized mice were stained with carboxyfluoresceinsuccinimidyl ester (CFSE) as described before [31] (link). Appropriate peptides (5 µg/ml) were added to cells plated in complete RPMI medium in 96-well plates and harvested after 5 days, washed with PBS and stained with anti-CD4-PE antibody (Southern Biotech). Phytohemagglutinin (PHA) was used as the positive control. Proliferation data was acquired on FACSCalibur (BD Biosciences) and CFSE-dilution in live CD4+ cells was analyzed using FlowJo (Tree Star).
Kashi V.P., Jacob R.A., Shamanna R.A., Menon M., Balasiddaiah A., Varghese R.K., Bachu M, & Ranga U. (2014). The Grafting of Universal T-Helper Epitopes Enhances Immunogenicity of HIV-1 Tat Concurrently Improving Its Safety Profile. PLoS ONE, 9(12), e114155.
+ Open protocol
+ Expand
2

Chicken Splenic T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
On 21 d after the second immunization, chicken splenic lymphocyte (n = 5) were collected and stained with anti-Chicken -CD3-FITC antibody, anti-CD4-PE antibody and anti-CD8A-APC antibody (Southern Biotech) according to a previous study (Bo et al., 2018 (link)). The expression of T cell differentiation was analyzed by the flow cytometer (BD Accuri C6).
Wusiman A., Zhang Y., Ayihengbieke T., Cheng X., Kudereti T., Liu D., Wang D, & Abula S. (2022). Poly (lactic-co-glycolic acid) nanoparticle-based vaccines delivery systems as a novel adjuvant for H9N2 antigen enhance immune responses. Poultry Science, 101(5), 101791.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!