Erlotinib, NVP-AEW541 and afatinib were kindly provided by OSI Pharmaceuticals (Farmingdale, USA), Novartis (Basel, Switzerland) and Boehringer Ingleheim (Vienna, Austria), respectively. Paclitaxel and gemcitabine were acquired from Sigma-Aldrich (Dorset, UK) and Healthcare at Home (UK), respectively, while Iressa (gefitinib) and crizotinib were purchased from Tocris (Avonmouth, UK), respectively. Lapatinib, sapitinib, canertinib, neratinib, imatinib and dasatinib were all acquired from Selleckchem (USA).
Primary and secondary antibodies for flow cytometry were purchased from R&D Systems (Abingdon, UK), while the secondary FITC-conjugated rabbit anti-mouse mAb STAR9B was purchased from AbD Seroctec (Kidlington, UK). Primary antibodies for western blot were obtained from Cell Signaling Technology Inc. (Massachusetts, USA), apart from the anti-EGFR antibody F4, which was obtained from Sigma-Aldrich, while secondary antibodies were purchased from Li-Cor Inc (Nebraska, USA).
Primary and secondary antibodies for flow cytometry were purchased from R&D Systems (Abingdon, UK), while the secondary FITC-conjugated rabbit anti-mouse mAb STAR9B was purchased from AbD Seroctec (Kidlington, UK). Primary antibodies for western blot were obtained from Cell Signaling Technology Inc. (Massachusetts, USA), apart from the anti-EGFR antibody F4, which was obtained from Sigma-Aldrich, while secondary antibodies were purchased from Li-Cor Inc (Nebraska, USA).