The heart rate measurements in this study were performed based on a previous study [35 (link)]. Self-attaching bandages (1410 1404, 3M, USA) and disposable electrodes (G236, NIHON KOHDEN, JPN) and sponges (6 cm—5 cm—2.5 cm) were combined as shown in S1 Fig to allow the electrodes to be placed on the body surface of the dog without shaving. A sponge was sandwiched between them firmly to fix the electrodes so that they would not float, and noise would enter. An ELECTRODE GEL (15–69, Parker Laboratories, USA) was also added to the electrodes. Faros (emotion Faros 360°, Bittium, Finland) was used as a device for recording the electrocardiogram of dogs and was finally attached as shown in S2 Fig . If the dog was unwilling to wear the electrocardiograph (ECG), the ECG was discontinued. All the dogs were not reluctant to wear the ECG.
Electrode gel
Manufactured by Parker Laboratories
Sourced in United States
Electrode gel is a conductive medium used to facilitate the transmission of electrical signals between the skin and electrodes in various medical and research applications. It enhances the contact and reduces the impedance between the skin and electrodes, improving the quality of signal acquisition and recording.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Hypoxanthine, by Thermo Fisher Scientific (1 mentions)
Agarose, by Merck Group (1 mentions)
Hoechst 33342 trihydrochloride trihydrate, by Thermo Fisher Scientific (1 mentions)
Dl lactic acid, by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide, by Merck Group (1 mentions)
Rabbit anti histamine, by Immunostar (1 mentions)
Vectashield plus, by Vector Laboratories (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
1 octen 3 ol, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions)
Shock generator, by Med Associates (1 mentions)
Env 610m, by Med Associates (1 mentions)
Hp7890 gc, by Agilent Technologies (1 mentions)
Cs 55, by Syntech (1 mentions)
Hp 5ms ui column, by Agilent Technologies (1 mentions)
5 protocols using electrode gel
1
Canine Electrocardiogram Measurement Protocol
2
Histamine Interaction with Red Blood Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Histamine (2-(1H-imidazol-5-yl)ethanamine; dihydrochloride, ACROS Organics, Fair Lawn, NJ, USA), human red blood cells (RBCs, Interstate Blood Bank, Memphis, TN, USA), and serum (Interstate Blood Bank, Memphis, TN, USA), RPMI-1640 medium (Gibco, Gaithersburg, MD, USA) supplemented with HEPES, L-glutamine, hypoxanthine (Acros Organics), DL-lactic acid (Acros Organics), Hoechst 33342-trihydrochloride trihydrate (Invitrogen, Carlsbad, CA, USA), JC-1 (Invitrogen, Carlsbad, CA, USA), mercurochrome, Ringer’s solution (3 mM CaCl2, 182 mM KCl, 46 mM NaCl, 10 mM Tris pH 7.2), 1-octen-3-ol (Sigma-Aldrich), and electrode gel (Parker Laboratories, Fairfield, NJ, USA). N-3-dimethylaminopropyl-N′-ethylcarbodiimide (Sigma-Aldrich, St. Louis, MO, USA, cat. #03449), paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA, cat. #15710), agarose (Sigma-Aldrich), goat serum (BSA; Jackson ImmunoResearch Laboratories, West Grove, PA, USA, cat. #001-000-162), rabbit anti-histamine (ImmunoStar, Hudson, WI, USA, cat. # 22939, RRID:AB_572245), Alexa Fluor 488 (Thermo Fisher, cat. #A-11008), and Vectashield®PLUS (Vector Laboratories, Burlingame, CA, cat. #H-1900).
3
Chemical Stimuli Delivery and Electrophysiological Recordings of Insect Chemoreception
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals for chemoreception studies were dissolved in mineral oil in a serial dilution ranging from 10-1 to 10-5 g/mL. Chemical delivery has been described earlier (Tait et al., 2016 (link)).
Animal preparation for electroantennography (EAG) and single sensillum recording (SSR) for Drosophila melanogaster and Apis dorsata has been described previously (Pellegrino et al., 2010 (link); Tait et al., 2016 (link)). EAGs with other insects were conducted using excised heads or antennae connected to a forkhead electrode (Ockenfels Syntech, Kirchzarten, DE) using electrode gel (Parker Laboratories, INC, NJ, United States). Data acquisition was performed using EAG2000 software (Ockenfels Syntech, Kirchzarten, DE). Protocol for SSR was as described (Pellegrino et al., 2010 (link); Tait et al., 2016 (link)) for all species tested (Drosophila melanogaster, Rhagoletis pomonella, and Helicoverpa armigera). Stimuli were presented as 0.5 s pulses at 0.5 L/min using a custom built stimulus delivery setup as described in Tait et al. (2016) (link). Syntech intelligent data acquisition controller (IDAC-4) and Autospike software (Ockenfels Syntech, Kirchzarten, DE) were used for SSR data acquisition.
Animal preparation for electroantennography (EAG) and single sensillum recording (SSR) for Drosophila melanogaster and Apis dorsata has been described previously (Pellegrino et al., 2010 (link); Tait et al., 2016 (link)). EAGs with other insects were conducted using excised heads or antennae connected to a forkhead electrode (Ockenfels Syntech, Kirchzarten, DE) using electrode gel (Parker Laboratories, INC, NJ, United States). Data acquisition was performed using EAG2000 software (Ockenfels Syntech, Kirchzarten, DE). Protocol for SSR was as described (Pellegrino et al., 2010 (link); Tait et al., 2016 (link)) for all species tested (Drosophila melanogaster, Rhagoletis pomonella, and Helicoverpa armigera). Stimuli were presented as 0.5 s pulses at 0.5 L/min using a custom built stimulus delivery setup as described in Tait et al. (2016) (link). Syntech intelligent data acquisition controller (IDAC-4) and Autospike software (Ockenfels Syntech, Kirchzarten, DE) were used for SSR data acquisition.
4
Primate Cognitive Behavior Testing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experimental sessions were conducted in ventilated and sound-attenuating chambers. Monkeys were seated in custom-made primate chairs (Crist Instrument Co., Hagerstown, MD). One response lever (model ENV-610M, Med Associates, Inc., Georgia, VT) was mounted on the wall of the chamber in front of the monkey. Each press of a lever with a minimum downward force of approximately 0.25 N produced an audible click and was recorded as a response. Food pellets (Formula 05474, 1 g, Bioserve, Frenchtown, NJ) could be delivered to a tray located between the levers. Colored lights mounted above the levers could be illuminated to serve as visual stimuli. Child-size shoes were fitted with brass electrode plates and were connected to a shock generator (Med Associates; Georgia, VT). Electrode gel (Parker Laboratories, Inc.; Fairfield, NJ) was applied to the plates to facilitate contact between the plates and the monkeys’ feet.
5
Moth Antenna Response to Volatile Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Electroantennogram (EAG) responses of male and female antennae to gland extracts and to volatile collections were measured using a GC-EAD system with an HP7890 GC (Agilent Technologies, Santa Clara, CA, USA) equipped with an HP-5MS UI column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies). The effluent air (from the odor source) was divided in a 1 : 1 ratio between a flame ionization detector (FID) and the EAD. An air stimulus controller (CS-55; Syntech, Hilversum, the Netherlands) was used for gas delivery. Tips of antennae of 1-to 2-d-old virgin males were cut off, and the head of a male moth with antennae was at-tached to the fork electrode with an electrode gel (Parker Laboratories, Inc., Fairfield, NJ, USA). Helium was used as carrier gas at a flow rate of 1.0 mL/min, and the injector temperature was set at 250 °C. The oven temperature was maintained at 50 °C for 1 min after injection and then increased by 5 °C/min to 200 °C with a hold for 1 min, then increased by 10 °C/min to 300 °C, with a final hold of 1 min. Recordings were made using the GC-EAD Pro ver. 4.1 software (Syntech).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!