HSCs were cultured in media composed of F12 media, 1% ITSX, 1%, 10 mM HEPES, 1% P/S/G, 100 ng/ml mouse TPO, 10 ng/ml mouse SCF and 0.1% of one the following chemicals (all from Sigma): hydroxypropyl cellulose (HPC; 435007), low-viscosity carboxymethylcellulose sodium salt (CMC-LV; C5678), medium viscosity carboxymethylcellulose sodium salt (CMC-MV; 21902), alpha-cyclodextrin (alpha-CD; C4642), beta-cyclodextrin (beta-CD; C4767), gamma-cyclodextrin (gamma-CD; C4930), 2-hydroxypropyl-betal—cylodextrin (HBC; H107), 2-hydroxypropyl-gamma-cycldextrin (HGC; H125), methyl-beta-cylodextrin (MBC; C4555), poloxamer 188 (polox188; P5556), or polyvinyl alcohol (PVA; P8136, 363081, or 363146), at 37°C with 5% CO2. For long-term cultures in PVA-based cultures, complete media changes were made every 2–3 days after the first 5–6 days, as described above for albumin-based cultures. Long-term cultures were split 1:3 at ~90% confluency. Where indicated, lipopolysaccharide (LPS; Sigma L2762) or IL-6 (Peprotech) were added to cultures.
Mouse scf
Manufactured by Merck Group
Mouse SCF is a recombinant protein that is a key regulator of hematopoiesis and melanogenesis. It functions as a growth factor and is critical for the survival, proliferation, and differentiation of various cell types, including hematopoietic stem cells, mast cells, and melanocytes.
Automatically generated - may contain errors
Lab products found in correlation
Mouse tpo, by Merck Group (4 mentions)
Hydroxypropyl cellulose, by Merck Group (2 mentions)
Alpha cyclodextrin, by Merck Group (2 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions)
Cell culture products, by Thermo Fisher Scientific (2 mentions)
Heparin, by Merck Group (2 mentions)
Small molecule inhibitors, by Cayman Chemical (2 mentions)
Human igf 2, by Merck Group (2 mentions)
Mouse fgf 1, by Merck Group (2 mentions)
Stemspan, by STEMCELL (2 mentions)
4 protocols using mouse scf
1
Expansion of Hematopoietic Stem Cells
2
Expansion of Hematopoietic Stem Cells
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HSCs were cultured in media composed of F12 media, 1% ITSX, 1%, 10 mM HEPES, 1% P/S/G, 100 ng/ml mouse TPO, 10 ng/ml mouse SCF and 0.1% of one the following chemicals (all from Sigma): hydroxypropyl cellulose (HPC; 435007), low-viscosity carboxymethylcellulose sodium salt (CMC-LV; C5678), medium viscosity carboxymethylcellulose sodium salt (CMC-MV; 21902), alpha-cyclodextrin (alpha-CD; C4642), beta-cyclodextrin (beta-CD; C4767), gamma-cyclodextrin (gamma-CD; C4930), 2-hydroxypropyl-betal—cylodextrin (HBC; H107), 2-hydroxypropyl-gamma-cycldextrin (HGC; H125), methyl-beta-cylodextrin (MBC; C4555), poloxamer 188 (polox188; P5556), or polyvinyl alcohol (PVA; P8136, 363081, or 363146), at 37°C with 5% CO2. For long-term cultures in PVA-based cultures, complete media changes were made every 2–3 days after the first 5–6 days, as described above for albumin-based cultures. Long-term cultures were split 1:3 at ~90% confluency. Where indicated, lipopolysaccharide (LPS; Sigma L2762) or IL-6 (Peprotech) were added to cultures.
3
Culture and Expansion of HSPCs
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293T cells were cultured in DMEM (high glucose) medium containing 10% FBS. K562 cells were cultured in IMDM medium plus 10% FBS. Enriched fresh hematopoietic stem and progenitor cells (HSPCs) were cultured in vitro in Stemspan (STEMCELL Technologies) supplemented with 10 μg/ml heparin (Sigma), 10 ng/ml mouse SCF, 20 ng/ml mouse TPO, 20 ng/ml human IGF-II, 10 ng/ml mouse FGF-1, and 100 ng/ml human Angptl3 25 . All recombinant proteins were purchased from PEPROTECH or PROSPEC companies. All cell culture products were purchased from Life Technologies unless otherwise mentioned. All small molecule inhibitors were purchased from Cayman Chemicals.
4
Culture and Expansion of HSPCs
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