Cells were lysed using lysis buffer (50 mM HEPES (pH 7.5), 100 mM NaCl, 50 mM KCl, 1% Triton X-100, 5% glycerol, 0.5% NP-40, 2 mM MgCl2, 1 μM DTT, cocktail and PMSF) followed by sonication and centrifugation at 4 °C. Extracts were quantified using the BCA method. Proteins were run on 4-12% SurePAGE-MOPS acrylamide gels (Genscript, China) and transferred onto PVDF membranes. Blots were blocked with 0.5% nonfat dry milk and incubated overnight at 4 °C with primary antibodies. Secondary antibodies were incubated for 1.5 h at room temperature, and then blots were developed with chemiluminescent reagents. Images were acquired with FluroChem M1 (Protein Simple). Uncropped scans were provided in the Source Data file.
The antibodies used for western blot were: anti-YAP/TAZ (Santa Cruz Biotechnology, sc-101199 (1:1000)), anti-YAP1 antibody (Proteintech, Cat#13584-1-AP (1:300)), anti-WWTR1 antibody (ATLAS, Cat#HPA007415 (1:300)) and anti-ADM antibody (Abcam, Cat#ab190819 (1:300)) and anti-GAPDH (Millipore, MAB374 (1:30000)). The secondary antibodies were from Beyotime Biotechnology (anti-mouse: Cat# A0216 (1:500), anti-rabbit: Cat# A0208 (1:500)).
The antibodies used for western blot were: anti-YAP/TAZ (Santa Cruz Biotechnology, sc-101199 (1:1000)), anti-YAP1 antibody (Proteintech, Cat#13584-1-AP (1:300)), anti-WWTR1 antibody (ATLAS, Cat#HPA007415 (1:300)) and anti-ADM antibody (Abcam, Cat#ab190819 (1:300)) and anti-GAPDH (Millipore, MAB374 (1:30000)). The secondary antibodies were from Beyotime Biotechnology (anti-mouse: Cat# A0216 (1:500), anti-rabbit: Cat# A0208 (1:500)).