For antibody purification from hybridoma supernates, HiTrap MabSelectSure columns (GE Healthcare Life Sciences) were used to purify antibodies using the manufacturer's protocol. To obtain fragment antigen-binding (Fab) fragments, papain digestion was used (Pierce Fab Preparation Kit, Thermo Scientific). Fab fragments were purified by removing IgG and Fc contaminants using a HiTrap MabSelectSure column followed by purification with an anti-CH1 column (GE Healthcare Life Sciences).
Anti ch1 column
Manufactured by GE Healthcare
The Anti-CH1 column is a chromatography column used for the purification of antibodies. It is designed to selectively bind to the CH1 domain of the antibody's constant region, allowing for the separation and isolation of the antibody from other components in a sample. The column's core function is to provide an efficient and reliable method for antibody purification, a crucial step in various biomedical and research applications.
Automatically generated - may contain errors
2 protocols using anti ch1 column
1
Antibody Purification and Fab Preparation
2
Antibody Purification and Fab Preparation
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