Tygon poly vinyl chloride pvc tubing

Materials for synthesis of the argatroban polymer solution were obtained from the following: CarboSil^® polymer from DSM Biomedical (Berkeley, CA); anhydrous hexane from Fisher Scientific (Fair Lawn, NJ); anhydrous tetrahydrofuran (THF), anhydrous hexamethylene diisocyanate (HMDI), argatroban monohydrate ((2F,4R)-1-[(2S)-5-(diaminomethylideneamino)-2-[[(3R)-3-methyl-1,2,3,4-tetrahydroquinolin-8-yl]sulfonylamino]pentanoyl]-4-methyl-piperidine-2-carboxylic acid) all from Sigma-Aldrich (St. Louis, MO). Hanks Balanced Salt Solution (HBSS) with calcium and magnesium was purchased from Gibco^® (Grand Island, NY). Materials for the chromogenic antithrombin assay were obtained from the following: chromogenic substrate (N-p-tosyl-Gly-Pro-Arg-p-nitroanilide acetate) from Sigma-Aldrich (St. Louis, MO); BC thrombin from Siemens (Newark, DE). ECC circuits were constructed from the following: Tygon^® poly(vinyl chloride) (PVC) tubing from Fisher Healthcare (Houston, TX); polyurethane angiocatheters, 16-gauge and 14-gauge, from Kendall Monoject Tyco Healthcare (Mansfield, MA).

Tygon® poly(vinyl chloride) (PVC) tubing was purchased from Fisher Healthcare (Houston, TX). Tetrahydrofuran (THF) was distilled over sodium and benzophenone prior to use. (Z)- 1 -[N-Butyl-N-[6[(N-butylammoniohexl)amino]]-diazen-1-ium-1,2-diolate (diazeniumdiolated dibutylhexanediamine (DBHD/N₂O₂)) was synthesized by treating N,N′-dibutyl-1,6-hexanediamine (American Custom Chemicals Corp., San Diego, CA) with nitric oxide (NO) (80 psi) at room temperature for 17–24 h as previously described^{20 (link)}. The following antibodies were purchased from BioRad (Hercules, CA): mouse anti-pig CD61 (IIIa subunit of GPIIb/IIIa) FITC, mouse anti-human P-selectin glycoprotein (CD62P) PE, mouse isotype controls for IgG1 FITC and IgG1 PE rat anti-mouse integrin Alexa Flour 488, rat anti-mouse CD11b Alexa Flour 488, rat anti-mouse CD14 Alexa Flour 488, mouse anti-human CD14 PE, rat isotype control for IgG2b Alexa Fluor 488, and mouse isotype control for IgG2a PE.The antibody clones for P-selectin, IIb/IIIa receptor, CD11b and CD14 have been previously shown to have cross-reactivity to the rabbit^{21 (link),22 (link)}.