Rankl elisa kits

TSG (purity = 94.7%) was obtained from the National Institutes for Food and Drug Control (Beijing, China). Fetal bovine serum (FBS) and alpha modification of Eagle’s minimum essential medium (α-MEM) medium were purchased from Hyclone (Logan, UT, USA). Methyl thiazolyl tetrazolium (MTT) and LY-294002 were purchased from Sigma Aldrich (St. Louis, MO, USA). The total RNA extraction kit was purchased from Aidlab (Beijing, China). The cell cycle analysis kit and materials for Western blot were purchased from Beyotime Biotechnology (Nantong, Jiangsu, China). Reverse transcriptase and qPCR Mix were purchased from TransGen Biotech (Beijing, China). Akt1 antibody was purchased from Signalway Antibody (College Park, MD, USA). PAkt1 (ser473) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). P-PI3K (p85) antibody was purchased from EnoGene (Nanjing, China). PI3K (p85), M-CSF, Osx, and Col1a1 antibodies were purchased from Wanleibio (Shenyang, China). The Runx2 antibody was purchased from Cusabio (Wuhan, China). GAPDH, β-actin, and β-tublin antibodies were purchased from Abclonal (Wuhan, China). OPG and RANKL ELISA kits were purchased from R&D Systems (Minneapolis, MN, USA).