In order to assess the interactions between PR and compositions of NLCs, FT-IR analysis was performed on PR only, lipid melt, PR–lipid melt, freeze-dried blank-NLCs, and freeze-dried PR-NLCs. To prepare lipid melt, GMS and olive oil were blended under stirring at 75 °C and then cooled in an ice bath. In the case of PR–lipid melt, it was prepared in the same method with PR. The spectra were recorded using a Thermo Scientific Nicolet 380 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). A total of 64 scans were used and data were recorded over the range 4000–400 cm–1 at room temperature. Background scanning and correction were performed before each measurement.
Nicolet 380 spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Nicolet 380 spectrophotometer is a laboratory instrument designed for the analysis and identification of various samples. It utilizes infrared light to capture the absorption spectrum of a sample, providing information about its molecular composition. The Nicolet 380 is capable of measuring the infrared absorption of solid, liquid, and gaseous samples, making it a versatile tool for a wide range of applications.
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Lab products found in correlation
Avance 400, by Bruker (1 mentions)
Gcms qp2010 ultra instrument, by Shimadzu (1 mentions)
Av 3 600 nmr spectrometer, by Bruker (1 mentions)
Apex ultra 7.0 t, by Bruker (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
5 protocols using nicolet 380 spectrophotometer
1
FT-IR Analysis of PR Interactions
2
Molecular Structures of PG and NFs
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The molecular
structures of PG and NFs of the three groups, PCL NFs (PCL NF), PCL
NFs containing 11% PG (PCL–PG11 NF), and PCL NFs containing
18% PG (PCL–PG NF18), were studied with the aid of FTIR (Thermo
Fisher Scientific Nicolet 380 Spectrophotometer, USA) at 4000–400
cm–1.
structures of PG and NFs of the three groups, PCL NFs (PCL NF), PCL
NFs containing 11% PG (PCL–PG11 NF), and PCL NFs containing
18% PG (PCL–PG NF18), were studied with the aid of FTIR (Thermo
Fisher Scientific Nicolet 380 Spectrophotometer, USA) at 4000–400
cm–1.
3
V(IV) Sorption by Polysaccharide Matrix
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The sorption of V(IV) by (PGA) were carried out at pH 3.0, 4.0, 4.5, 5.0 and 6.0 by dipping the polysaccharidic matrix (25 mg), into 100 mL of solutions containing 12 μmol of V(IV) (120 μM). The amount of V(IV) sorbed was quantified by the difference between the initial concentration and that found at equilibrium. The FT-IR spectra of the V(IV)-PGA samples, centrifuged at 5000 rpm (5°C), washed several times with water, dehydrated and stored under vacuum, were recorded on KBr disks (2 mg of sample in 100 mg of KBr) with a Nicolet 380 spectrophotometer (Thermo Fisher Scientific).
4
Synthesis of Novel Organic Compounds
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The solvents and chemicals were procured from commercial chemical suppliers (Merck.KGaA, 64271 Darmstadt, EDM Millipore Corporation (Germany), Alfa Aesar, 30 Bond Street, Ward Hill) and used without further purification. Melting points were recorded using an Electrothermal IA-9100 digital fuser. Thermo Scientific Nicolet 380 spectrophotometer was used for FTIR spectra. 1H (400 MHz) and 13C NMR (100 MHz) spectra were recorded on Bruker Avance 400 instrument in DMSO‑d6. HRMS spectra were recorded using the Shimadzu GCMS-QP2010 Ultra instrument. All reactions were monitored by TLC performed on silica gel 60 F254 plates. The detail of synthesis experiments are given in the Supplementary data.
5
Synthesis and DNA Interaction of Pt(II) Complexes
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All reagents and chemicals were purchased from commercial sources and used as received. Salicylaldehyde and K 2 PtCl 4 were Chemical Grade, 2amino pyridine and 2-pyridine methanamine were analytical grade, MTT, and salmon sperm DNA were from Sigma. Three different human carcinoma cell lines: A549, Hela, and MCF-7 are obtained from American Type Culture Collection.
Instrumentation and measurement. Elemental analysis was determined on an Exeter Analytical CE-440 elemental analyzer. The IR spectra were recorded using KBr tablets and a Thermo Nicolet 380 spectrophotometer. The 1 H NMR spectra were recorded in DMSO-d 6 on a Bruker AVIII 600 NMR spectrometer. The mass spectra were measured by Bruker apex-ultra 7.0T. The interaction between DNA and complexes was measured on UV-3400 Toshniwal spectrophotometer and Brucker advance II GT-34 CD spectroscopy. Molar conductances were measured at room temperature in 1 × 10 -3 M methanol using a DDS-12DW-type conductivity meter.
Instrumentation and measurement. Elemental analysis was determined on an Exeter Analytical CE-440 elemental analyzer. The IR spectra were recorded using KBr tablets and a Thermo Nicolet 380 spectrophotometer. The 1 H NMR spectra were recorded in DMSO-d 6 on a Bruker AVIII 600 NMR spectrometer. The mass spectra were measured by Bruker apex-ultra 7.0T. The interaction between DNA and complexes was measured on UV-3400 Toshniwal spectrophotometer and Brucker advance II GT-34 CD spectroscopy. Molar conductances were measured at room temperature in 1 × 10 -3 M methanol using a DDS-12DW-type conductivity meter.
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