3h estrone 3 sulfate e3s

[³H]Estrone-3-sulfate (E3S; specific activity 57.3 Ci/mmol) was purchased from PerkinElmer (Melbourne, VIC, Australia). Dulbecco's modified Eagle's medium (DMEM) was obtained from Thermo Scientific (Lidcombe, NSW, Australia). Puromycin was purchased from Sapphire Biosciences (Redfern, NSW, Australia). Endo F and Endo Hf were purchased from Genesearch (Arundel, Qld, Australia). Protease inhibitor cocktail tablets were obtained from Roche Diagnostics Australia Pty. Ltd. (Castle Hill, NSW, Australia). Anti-flag and anti-myc antibodies were from Genesearch, the horseradish peroxidase-conjugated goat anti-rabbit IgG was from Sapphire Biosciences and the anti-β-actin antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Unless otherwise stated, all other chemicals and biochemicals were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia).