P nf kb p65

Flag (1:1000), c-Myc (Santacruz-SC-40; 1:750 and sc-764; 1:1000), p62 (BD; 1:2000), Actin (Abcam; 1:5000), IRGM (Abcam; 1:500), HA (CST; 1:1000), IL-1β (CST; 1:1000), Caspase-1(Adipogen; 1:1000), NLRP3 (Adipogen; 1:1000), GFP (Abcam; 1:5000), ASC (Santacruz; 1:1000), ATG-5 (CST, 1:1000), ATG7 (CST, 1:1000), NLRC4 (CST; 1:1000) and AIM2 (CST; 1:1000), IRGM Antibody Rodent specific (CST; 1:1000), c-Myc (Santa Cruz; 1:750). Phsopho-p38 MAPK (CST, 1:1000), p-38α (Santa cruz, 1:1000), Anti-pro Caspase1+p10+p12 Antibody (Abcam; 1:1000), NF-kb p65 (Santa cruz; 1:1000), p- NF-kb p65 (Santa cruz; 1:1000).
HRP conjugated secondary antibodies were purchased from Santa cruz (1:2000) or Promega (1:5000) or Abcam (1:10000).

Mouse epidermal Cl41 cells and their transfectants were cultured in 5% Fetal bovine Serum (FBS) MEM containing with 1% penicillin/streptomycin and 2 mM L-glutamine (Life Technologies). Cells were maintained in a humidified incubator at 37°C with 5% CO₂ atmosphere[45 (link)]. The antibodies specific against phospho-c-Jun, c-Jun, phospho-JNKs, JNKs, phospho-ERKs, ERKs, phospho-p38, p38, phospho-MKK7, MKK7, phospho-MKK4, MKK4, PP2A A subunit, XIAP, SP1, β-Actin and GAPDH were purchased from Cell Signaling Technology Inc (Beverly, MA). The antibodies specific against cyclin D1, c-Fos, CREB, Fra1, p-NF-kB p65, NF-kB p50, JunD, JunB, PTEN and MKP-1 were bought from Santa Cruz Biotechnology (Santa Cruz, CA). The antibody against FLAG was obtained from Covance Inc. (Princeton, NJ). The antibodies against phospho-PP2A and PP2A B subunit were purchased from Epitomics (Burlingame, CA). Actinomycin D was purchased from Calbiochem (Billerica, MA). ISO with over 98% purity was purchased by Higher Biotech (Shanghai, China). The preparation of ISO was performed as described in our previous studies [12 (link)].