Rabbit anti β actin 4970

Manufactured by Cell Signaling Technology

Rabbit anti-β-actin #4970 is a primary antibody produced in rabbit that recognizes the beta-actin protein. It is intended for use in various immunological techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to detect and analyze the expression of beta-actin in biological samples.

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Lab products found in correlation

Mini protean tgx gel, by Bio-Rad (1 mentions) Passive lysis buffer (plb), by Promega (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Mouse anti α tubulin t5168, by Merck Group (1 mentions) Rabbit anti snap tag, by New England Biolabs (1 mentions) Rabbit anti β arrestin1 2 d24h9, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

β-actin (4970) Anti-actin (4970) Rabbit anti-β-actin Anti-β-actin Rabbit anti-actin β-actin Anti-actin

2 protocols using rabbit anti β actin 4970

Western Blot Analysis of Angiogenic Factors

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Protein was extracted from cells using Passive Lysis Buffer (Promega) and samples were loaded onto 4%-20% Mini-PROTEAN TGX gel (Bio-Rad Laboratories, Hercules, CA). The primary antibodies include rabbit anti-BCAR3 #A301-671A (Bethyl Laboratories, Montgomery, TX), ANG 1 (C-20): sc-1408 (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-β-actin #4970 (Cell Signaling Technologies, Danvers, MA). All westerns were repeated two or more times with similar results for each cell line and representative westerns with more than one cell line are included at full size in the Supplementary Figure 4.

Zhou K., Diebel K.W., Holy J., Skildum A., Odean E., Hicks D.A., Schotl B., Abrahante J.E., Spillman M.A, & Bemis L.T. (2017). A tRNA fragment, tRF5-Glu, regulates BCAR3 expression and proliferation in ovarian cancer cells. Oncotarget, 8(56), 95377-95391.

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Immunoblotting Protocol for Protein Detection

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Samples were fractioned by SDS–PAGE on 8% gels under reducing conditions, immunoblotted onto nitrocellulose membranes (GE Healthcare) and bands detected by enhanced chemiluminescence (GE Healthcare) onto films developed on a Xograph Compact X5 processor.
Antibodies were primaries rabbit anti-β-arrestin-1/2 (D24H9, Cell Signaling, 1/1000), rabbit anti-SNAP tag (New England Biolabs, 1/500), mouse anti-α-tubulin (T5168, Sigma, 1/1000), rabbit anti-β-actin (4970, Cell Signaling, 1/1000), and mouse anti-GAPDH (6C5, Merck, 1/10,000); and IgG-HRP secondaries (Santa Cruz Biotechnology).

Jones B., Buenaventura T., Kanda N., Chabosseau P., Owen B.M., Scott R., Goldin R., Angkathunyakul N., Corrêa Jr I.R., Bosco D., Johnson P.R., Piemonti L., Marchetti P., Shapiro A.M., Cochran B.J., Hanyaloglu A.C., Inoue A., Tan T., Rutter G.A., Tomas A, & Bloom S.R. (2018). Targeting GLP-1 receptor trafficking to improve agonist efficacy. Nature Communications, 9, 1602.

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