Tween 20 tbst

Manufactured by Thermo Fisher Scientific

Tween-20 (TBST) is a non-ionic surfactant commonly used in biochemical and molecular biology applications. It is a polyoxyethylene sorbitan monolaurate that helps to solubilize and stabilize proteins in aqueous solutions. Tween-20 is often used in buffer solutions, such as TBST (Tris-Buffered Saline with Tween-20), to facilitate protein-protein interactions and reduce non-specific binding during techniques like Western blotting and immunoassays.

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Lab products found in correlation

Protease inhibitor, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Bio-Rad (2 mentions) Dry milk, by Bio-Rad (2 mentions) Fluorchem imaging system, by Bio-Techne (2 mentions) Ecl substrate, by Bio-Rad (1 mentions)

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Tween-20 (331 citations)

2 protocols using tween 20 tbst

Quantifying CD166 Expression in Co-cultured Constructs

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Western blotting was performed on both aligned and random co-cultured constructs to assess the protein expression of CD166. Construct lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer with 1% protease inhibitors (Thermo Fisher). Denatured cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred onto a polyvinylidene fluoride (PVDF) membrane. Blocking was performed with Tris-buffered saline (TBS) supplemented with 0.1% Tween-20 (TBST) (Thermo Fisher Scientific, Waltham, MA) and 5% dry milk (Bio-rad). The blocked membrane was incubated with the primary anti-CD166 antibody (Abcam) overnight at 4 °C. Horseradish peroxidase-conjugated secondary antibody (1:3000, Bio-rad) and enhanced luminol-based chemiluminescent (ECL) substrate (Bio-rad) was used to develop the bands. The developed membrane was imaged using a FluorChem Imaging System (Alpha InnoTech, San Leandro, CA).

Qian Z., Sharma D., Jia W., Radke D., Kamp T, & Zhao F. (2019). Engineering stem cell cardiac patch with microvascular features representative of native myocardium. Theranostics, 9(8), 2143-2157.

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Quantifying Growth Factors in Prevascularized Constructs

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Western blotting was performed to compare the relative amounts of growth factors VEGFA, bFGF, as well as cell surface receptor VEGFR2 in cultured prevascularized constructs. Radioimmunoprecipitation assay (RIPA) buffer with 1% protease inhibitors (Thermo Fisher) was used to collect cell lysates. Cell lysates were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and transferred onto a polyvinylidene fluoride (PVDF) membrane. The membranes were blocked with TBS supplied with 0.1% Tween-20 (TBST) (Thermo Fisher Scientific, Waltham, MA) and 5% dry milk (Bio-rad) and then incubated with primary antibodies overnight at 4 °C. A horseradish peroxidase–conjugated secondary antibody (1:3000, Bio-rad) was then added after washing, and incubated for 1 hour at room temperature. An ECL substrate (Bio-rad) was used to acquire the chemiluminescent signal, which was imaged using a FluorChem Imaging System (Alpha InnoTech, San Leandro, CA).

Zhang L., Qian Z., Tahtinen M., Qi S, & Zhao F. (2017). Prevascularization of Natural Nanofibrous Extracellular Matrix for Engineering Completely Biological 3D Prevascularized Tissues for Diverse Applications. Journal of tissue engineering and regenerative medicine, 12(3), e1325-e1336.

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