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Sybr qpcr master mix

Manufactured by Transgene
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Sourced in China

SYBR qPCR Master Mix is a ready-to-use solution designed for quantitative real-time PCR (qPCR) analysis. It contains SYBR Green I dye, DNA polymerase, and all the necessary reagents for efficient and sensitive DNA quantification.

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Lab products found in correlation

Real time pcr detection system, by Bio-Rad (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Fastking rt kit, by Tiangen Biotech (1 mentions)

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SYBR qPCR Mix (4 citations)

2 protocols using sybr qpcr master mix

1

Isolation and Quantification of Total RNA

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Total RNA was isolated using the TRIzol reagent (Invitrogen, 15596018). The concentration of RNA was determined from spectrophotometric analysis at A260/280. First-strand cDNA was synthesized using FastKing RT Kit (TIANGEN, Beijing, China) at 42°C for 15 min. Polymerase chain reaction (PCR) amplification of the cDNA was performed using SYBR qPCR Master Mix (AQ211-01, TransGen Biotech, Beijing, China) on real-time PCR detection system (Bio-Rad, Hercules, CA, USA). 18S was used as an internal control.
Yan C.Y., Ye Y., Mu H.L., Wu T., Huang W.S., Wu Y.P., Sun W.Y., Liang L., Duan W.J., Ouyang S.H., Huang R.T., Wang R., Sun X.X., Kurihara H., Li Y.F, & He R.R. (2023). Prenatal hormone stress triggers embryonic cardiac hypertrophy outcome by ubiquitin-dependent degradation of mitochondrial mitofusin 2. iScience, 27(1), 108690.
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2

Quantitative Real-Time PCR for Gene Expression

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Quantitative real-time PCR (qRT-PCR) was conducted using the SYBR® qPCR Master Mix (TransGen Biotech, Beijing, China) on the LightCycler 480 real-time PCR instrument (Roche Diagnostics, Burgess Hill, United Kingdom). Amplification parameters refer to Wang et al., 2019 (link). The specific primers were listed in Supplementary Table S3.
Wang Z., Zhu S., Yin S., Zhao Z., Zheng Z, & Deng Y. (2022). DNA Methylation Analyses Unveil a Regulatory Landscape in the Formation of Nacre Color in Pearl Oyster Pinctada fucata martensii. Frontiers in Genetics, 13, 888771.
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