The following reagents were used: α-minimum essential medium, (α-MEM; 135-15175, WAKO, Osaka, Japan); fetal bovine serum (FBS; 10270, Gibco), anti-Arp3 antibody (sc-48344, 1:100, Santa Cruz Biotechnology); anti-β-actin antibody (ab8226, 1:100, Abcam); anti-non-muscle myosin heavy chain IIA antibody (PRB-440P-100, 1:100, BioLegend, Dedham, MA); anti-cortactin antibody [5-180, 1:100, Millipore (Upstate)]; anti-paxillin antibody (610051, 1:100, BD Biosciences); anti-vinculin antibody (V9264, 1:100, Sigma-Aldrich); anti-zyxin antibody (sc-6438, 1:100, Santa Cruz Biotechnology); rhodamine-phalloidin (R415, Molecular Probes, Thermo Fisher Scientific); fluorescein isothiocyanate-phalloidin (P5282, Sigma-Aldrich); DAPI (D1306, Molecular Probes, Thermo Fisher Scientific, CA); recombinant mouse receptor activator of nuclear factor kappa-Β (sRANK) ligand (315-11, PeproTech, NJ); fluorescein isothiocyanate- and tetramethylrhodamine isothiocyanate-conjugated secondary antibodies (715-095-151, 711-095-152 and 712-096-153, Jackson ImmunoResearch, PA); CK-666 (182515, Calbiochem, Merck Millipore, Darmstadt, Germany) and ML-7 (475880, Calbiochem).
Anti vinculin antibody v9264
Manufactured by Merck Group
Sourced in Morocco
The Anti-vinculin antibody (V9264) is a laboratory reagent used for the detection and localization of vinculin, a cytoskeletal protein, in various cell and tissue samples. This antibody can be utilized in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions)
Phalloidin fluorescein isothiocyanate, by Merck Group (1 mentions)
Anti cortactin antibody, by Merck Group (1 mentions)
Anti β actin antibody ab8226, by Abcam (1 mentions)
Dapi d1306, by Thermo Fisher Scientific (1 mentions)
α mem, by Fujifilm (1 mentions)
2 protocols using anti vinculin antibody v9264
1
Cytoskeletal Dynamics in Cell Adhesion
2
Immunofluorescence Staining of Focal Adhesions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neurons were fixed with 4% paraformaldehyde (in PBS) for 20 min at room temperature and then incubated with 0.25% Triton X-100 (in PBS) and 1% BSA (in PBS) for 1 h for permeabilization and blocking. Primary antibodies (FAK [pTyr397] antibody, 700255, Invitrogen; Paxillin [pY118] antibody, 44722G, Invitrogen; anti-vinculin antibody, V9264, Sigma-Aldrich; Phospho-Myosin Light Chain 2 [Ser19] Mouse mAb, 3657, Cell Signaling Technology; anti-green fluorescent protein, A11120 , Molecular Probes) were added with corresponding blocking solution and incubated overnight at 4°C. Corresponding secondary antibodies and DAPI were added and incubated for 2 h at room temperature.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!