Ebc 1

Manufactured by RIKEN BioResource Center

Sourced in Japan

The EBC-1 is a laboratory equipment designed for the extraction and purification of biomolecules from various biological samples. It utilizes an electric field-based system to efficiently separate and collect targeted biomolecules. The core function of the EBC-1 is to provide a reliable and reproducible method for the isolation of proteins, nucleic acids, and other macromolecules from complex biological matrices.

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Lab products found in correlation

Thp 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Hyclone, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Jurkat, by American Type Culture Collection (1 mentions) Molt 4, by American Type Culture Collection (1 mentions) Hsc 3, by RIKEN BioResource Center (1 mentions) Hsc 4, by RIKEN BioResource Center (1 mentions) Lc 1 sq, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Ham s f12, by Thermo Fisher Scientific (1 mentions) Rerf lc ai, by RIKEN BioResource Center (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)

3 protocols using ebc 1

Cell Culture Techniques for LUSC and THP-1

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Cell lines of human LUSC, H226 (ATCC) and EBC‐1 (Riken BioResource Center), and human monocyte, THP‐1 (Japanese Collection of Research Bioresources Cell Bank), were obtained and cultured in RPMI 1640 medium (Sigma‐Aldrich) supplemented with 10% heat‐inactivated FBS (HyClone, Thermo Fisher Scientific K.K.) and penicillin/streptomycin in a humidified atmosphere with 5% CO₂ at a constant temperature of 37°C. All cell lines were tested for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza), and mycoplasma negativity was confirmed before use.

Sumitomo R., Menju T., Shimazu Y., Toyazaki T., Chiba N., Miyamoto H., Hirayama Y., Nishikawa S., Tanaka S., Yutaka Y., Yamada Y., Nakajima D., Ohsumi A., Hamaji M., Sato A., Yoshizawa A., Huang C., Haga H, & Date H. (2023). M2‐like tumor‐associated macrophages promote epithelial–mesenchymal transition through the transforming growth factor β/Smad/zinc finger e‐box binding homeobox pathway with increased metastatic potential and tumor cell proliferation in lung squamous cell carcinoma. Cancer Science, 114(12), 4521-4534.

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HNSCC and Lung Carcinoma Cell Lines

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Four human HNSCC cell lines, HSC3 (tongue SCC, HLA-DR15), HSC4 (tongue SCC, HLA-DR1/4, 53), Sa-3 (gingival SCC, HLA-DR9/10, 53) and SAS (tongue SCC, HLA-DR9/15, 53); two human lung carcinoma cell lines, Lu65 (large cell carcinoma, HLA-DR4/15, 53) and EBC1 (lung SCC, HLA-DR1); and two human T-cell leukemia cell lines, Jurkat (HLA-DR negative) and Molt-4 were used in this study. HSC3, HSC4, Sa-3, Lu65 and EBC1 were supplied by the RIKEN BioResource Center (Tsukuba, Ibaraki, Japan). SAS, Jurkat and Molt-4 were purchased from the American Type Culture Collection (ATCC; Manassas, VA). Mouse fibroblast cell lines (L-cells) expressing individual human HLA-DR molecules (DR4, DR8, DR15 and DR53) were kindly provided by Dr. T. Sasazuki (Kyushu University, Fukuoka, Japan) and Dr. Robert W. Karr (Karr Pharma, St. Louis, MO). All cell lines were maintained in tissue culture as recommended by the supplier.

Hayashi R., Nagato T., Kumai T., Ohara K., Ohara M., Ohkuri T., Hirata-Nozaki Y., Harabuchi S., Kosaka A., Nagata M., Yajima Y., Yasuda S., Oikawa K., Kono M., Kishibe K., Takahara M., Katada A., Hayashi T., Celis E., Harabuchi Y, & Kobayashi H. (2020). Expression of placenta-specific 1 and its potential for eliciting anti-tumor helper T-cell responses in head and neck squamous cell carcinoma. Oncoimmunology, 10(1), 1856545.

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Human Lung SCC Cell Line Culture

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We purchased the human lung SCC cell lines LK-2, EBC-1, and RERF-LC-AI from Riken Bioresource Center (Tsukuba, Japan) and LC-1sq from the JCRB Cell Bank (Tokyo). The EBC-1 and RERF-LC-AI cells were cultured in MEM (Invitrogen-Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 U/ml streptomycin. The LK-2 cells were cultured in RPMI 1640 (Invitrogen-Gibco) supplemented with 10% FBS, 100 U/ml penicillin and 100 U/ml streptomycin. The LC-1sq cells were cultured with a 1:1 mixture of RPMI 1640 and Ham's F12 (Invitrogen-Gibco) supplemented with 10% FBS, 100 U/ml penicillin and 100 U/ml streptomycin.

Ozono K., Ohishi Y., Onishi H., Nakamura K., Motoshita J., Kato M., Nakanishi R., Nakamura M, & Oda Y. (2017). Brain-derived neurotrophic factor/tropomyosin-related kinase B signaling pathway contributes to the aggressive behavior of lung squamous cell carcinoma. Laboratory investigation; a journal of technical methods and pathology, 97(11).

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