ChIP assay was performed as previously described [15 (link)] with the following antibodies: 3 ug of anti-H3K9me3 (ab8898, abcam), 3 ug of anti-H4K20me3 (ab9053, abcam), 2 ug of anti-TRF2 (NB110–57130, Novus Biologicals), 5 ug of anti-HP-1ɣ (05–690, Sigma) and IgG (sc-2025, Santa Cruz Biotechnology). Samples were transferred to a Hybond-N+ membrane (Amersham) on a dot blot, and hybridized with the same telomeric probe (TTAGGG)n used for TRF. The signal was quantified with the ImageJ software. The amount of telomeric DNA in each ChIP was normalized to total telomeric DNA signal.
Anti hp 1
Manufactured by Merck Group
Anti-HP-1ɣ is a laboratory tool designed to detect and measure the presence of HP-1ɣ, a specific protein, in biological samples. It provides a reliable and accurate method for researchers and scientists to analyze the levels of this target protein as part of their investigations.
Automatically generated - may contain errors
2 protocols using anti hp 1
1
Chromatin Immunoprecipitation of Telomeric DNA
2
Chromatin Immunoprecipitation of Telomeric DNA
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ChIP assay was performed as previously described [15 (link)] with the following antibodies: 3 ug of anti-H3K9me3 (ab8898, abcam), 3 ug of anti-H4K20me3 (ab9053, abcam), 2 ug of anti-TRF2 (NB110–57130, Novus Biologicals), 5 ug of anti-HP-1ɣ (05–690, Sigma) and IgG (sc-2025, Santa Cruz Biotechnology). Samples were transferred to a Hybond-N+ membrane (Amersham) on a dot blot, and hybridized with the same telomeric probe (TTAGGG)n used for TRF. The signal was quantified with the ImageJ software. The amount of telomeric DNA in each ChIP was normalized to total telomeric DNA signal.
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