Sample diluent hb buffer

Manufactured by Bio-Rad

Sample Diluent HB buffer is a laboratory reagent used to dilute biological samples prior to analysis. It maintains the pH and ionic strength of the sample, helping to preserve the integrity of the analytes. The buffer composition is designed to be compatible with a variety of analytical techniques.

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Lab products found in correlation

Bio plex 200 system, by Bio-Rad (3 mentions) Pro human cytokine panel 17 plex assay, by Bio-Rad (3 mentions)

Spelling variants of this product

Sample buffer (188 citations) Sample Diluent (13 citations)

3 protocols using sample diluent hb buffer

Multiplex Cytokine Profiling in Plasma

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All plasma samples were diluted 4-fold in Bio-Plex Sample Diluent HB buffer. The plasma samples were analyzed via a Bio-Plex Pro Human Cytokine Panel 17-Plex assay per the manufacturer’s instructions. Plates were read using the Bio-Plex 200 system (Bio-Rad, Hercules, CA).

Tang J., Ravichandran S., Lee Y., Grubbs G., Coyle E.M., Klenow L., Genser H., Golding H, & Khurana S. (2021). Antibody affinity maturation and plasma IgA associate with clinical outcome in hospitalized COVID-19 patients. Nature Communications, 12, 1221.

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Multiplex Cytokine Quantification

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All plasma/serum samples were diluted fourfold in Bio-Plex Sample Diluent HB buffer. The plasma/serum samples were analyzed via a Bio-Plex Pro Human Cytokine Panel 17-Plex assay per the manufacturer’s instructions. Plates were read using the Bio-Plex 200 system (Bio-Rad, Hercules, CA).

Ravichandran S., Lee Y., Grubbs G., Coyle E.M., Klenow L., Akasaka O., Koga M., Adachi E., Saito M., Nakachi I., Ogura T., Baba R., Ito M., Kiso M., Yasuhara A., Yamada S., Sakai-Tagawa Y., Iwatsuki-Horimoto K., Imai M., Yamayoshi S., Yotsuyanagi H., Kawaoka Y, & Khurana S. (2021). Longitudinal antibody repertoire in “mild” versus “severe” COVID-19 patients reveals immune markers associated with disease severity and resolution. Science Advances, 7(10), eabf2467.

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Multiplex Cytokine Profiling of Biofluids

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All serum/nasal wash samples were diluted fourfold in Bio-Plex Sample Diluent HB buffer. The samples were analyzed via a Bio-Plex Pro Human Cytokine Panel 17-Plex assay as per the manufacturer’s instructions. Plates were read using the Bio-Plex 200 system (Bio-Rad, Hercules, CA). Additional nine cytokines included measurement of type 1 IFN were determined using the individual human cytokine kits (R&D Systems, Minneapolis, MN). The cytokine assay was performed in duplicate (two independent experiments by research fellow in the laboratory who was blinded to sample identity). Similar cytokine levels were observed in the two assays.

Ravichandran S., Grubbs G., Tang J., Lee Y., Huang C., Golding H, & Khurana S. (2021). Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2–infected individuals reveal unlinked immune signatures. Science Advances, 7(42), eabi6533.

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