Notch3 d11b8

Manufactured by Cell Signaling Technology

Sourced in United States

NOTCH3 (D11B8) is a rabbit monoclonal antibody that recognizes the Notch3 protein. Notch3 is a transmembrane receptor that plays a role in cell-cell communication and developmental signaling pathways.

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Lab products found in correlation

Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti mouse igg hrp, by Agilent Technologies (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Cleaved parp asp214, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp, by Agilent Technologies (1 mentions) Ccnd1, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Phospho mtor, by Cell Signaling Technology (1 mentions) Py1173 egfr, by Cell Signaling Technology (1 mentions) β tubulin antibody, by Merck Group (1 mentions) Egfr 1005, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Notch3 (12 citations)

2 protocols using notch3 d11b8

Comprehensive Western Blot Analysis

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Western blot was performed with whole-cell protein lysates in RIPA lysis buffer. Equal amounts of 10 µg proteins were used for western blot analyses. PHLDB2 primary antibody was from Abcam (1:1000, ab202350, Cambridge, UK). Other primary antibodies were from Cell Signaling (Danvers, MA, US), including NOTCH3 (D11B8) (1:1000, #5276), p21 (1:1000, #2946), p27 (1:1000, #2552), Phospho-Rb (Ser807/811) (1:1000, #9308), cleaved caspase-3 (Asp175) (1:1000, #9661), cleaved-PARP (Asp214) (1:1000, #9541), Phospho-Akt (Ser473) (1:1000, #4060), Phospho-mTOR (Ser2448) (1:1000, #5536), CCND1 (1:1000, #2978), CDK4 (1:1000, #12790), CDK6 (1:1000, #3136), and GAPDH (1:2000, #2118). Secondary antibodies anti-Mouse IgG-HRP (1:30000, 00049039) and anti-Rabbit IgG-HRP (1:10000, 00028856) were from Dako (Glostrup, Denmark).

Kang W., Zhang J., Huang T., Zhou Y., Wong C.C., Chan R.C., Dong Y., Wu F., Zhang B., Wu W.K., Chan M.W., Cheng A.S., Yu J., Wong N., Lo K.W, & To K.F. (2021). NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway. Oncogene, 40(9), 1578-1594.

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Antibody Immunoblotting Protocol

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Following antibodies were used in this study: EGFR (1005) Santa Cruz Biotechnology, EGFR (Ab12) and EGFR (Ab15) are from Neomarker, Notch1 (5B5), Notch3 (8G5), and Notch3 (D11B8) and EGFR (pY1173) obtained from Cell Signaling Technology. Mouse-anti phosphotyrosine is from BD Transduction Laboratories. β-tubulin antibodies were obtained from Sigma.

Arasada R.R., Amann J.M., Rahman M.A., Huppert S.S, & Carbone D.P. (2014). EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling. Cancer research, 74(19), 5572-5584.

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