Cd4 and cd8 antibodies

Manufactured by Thermo Fisher Scientific

The CD4 and CD8 antibodies are lab equipment used to identify and quantify T cell subpopulations. They are designed to bind to specific cluster of differentiation (CD) molecules expressed on the surface of T cells, allowing for the detection and enumeration of these cell types.

Automatically generated - may contain errors

Lab products found in correlation

Lsr fortessa cell sorter, by BD (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD4 and CD8 (6 citations) Monoclonal antibodies for CD4 and CD8 (3 citations) Anti-CD4 and anti-CD8 antibodies (2 citations)

2 protocols using cd4 and cd8 antibodies

Cytokine Profiling of Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cervical and axillary lymph nodes were collected from mice at euthanasia and the cells treated with Brefeldin-A for 2 hours in 37°C to arrest extra-cellular secretion of cytokines. Cells were then washed and stained with fluorescently labelled CD4 and CD8 antibodies (eBioscience) overnight. The cells were washed the following day, subjected to fixation and permeabilization for intracellular staining, and stained with fluorescently labelled antibodies to IFN-γ IL-10, IL-12, and TNF-α (eBioscience). Cells were washed, fixed in 1% paraformaldehyde in FACS buffer and analyzed on an LSR Fortessa cell sorter (BD Bioscience).

Honjo T., Chyu K.Y., Dimayuga P.C., Lio W.M., Yano J., Trinidad P., Zhao X., Zhou J., Cercek B, & Shah P.K. (2015). Immunization with an ApoB-100 Related Peptide Vaccine Attenuates Angiotensin-II Induced Hypertension and Renal Fibrosis in Mice. PLoS ONE, 10(6), e0131731.

+ Open protocol

+ Expand

T Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Coro 1A^−/− or WT T cells were labeled with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Invitrogen). Equal amounts of labeled Coro 1A^−/− or WT T_effs and B6D2 irradiated splenocytes were cultured in RPMI complete for 24 or 48 hours. Following harvest the cells were stained with CD4 and CD8 antibodies (eBioscience). Proliferation was determined by flow cytometry using the FlowJo analysis software.

Fulton L.M., Taylor N.A., Coghill J.M., West M.L., Föger N., Bear J.E., Baldwin A.S., Panoskaltsis-Mortari A, & Serody J.S. (2014). Altered T-Cell entry and egress in the absence of Coronin 1A attenuates murine acute graft versus host disease. European journal of immunology, 44(6), 1662-1671.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!