Anti ku80 c 20

Manufactured by Santa Cruz Biotechnology

Anti-KU80 (C-20) is an antibody that targets the KU80 protein. KU80 is a subunit of the Ku heterodimer, which plays a crucial role in the repair of DNA double-strand breaks through the non-homologous end-joining pathway. This antibody can be used in various research applications to detect and analyze the KU80 protein.

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Lab products found in correlation

Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti actin 1 19, by Santa Cruz Biotechnology (1 mentions) Anti chk1 ps317, by Cell Signaling Technology (1 mentions) Anti dna pkcs ps2056, by Abcam (1 mentions) Anti chk1 g 4, by Santa Cruz Biotechnology (1 mentions) Species specific hrp conjugated secondary antibodies, by Santa Cruz Biotechnology (1 mentions) Supersignal west pico substrate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-Ku80 (4 citations) Ku80 (C-20, (2 citations)

2 protocols using anti ku80 c 20

Cell Lysis and Antibody Analysis

Cited 1 time

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Cells were lysed as previously described [30] (link). The following antibodies that follow were used: anti-Tag (Pab101, [84] (link)), anti-actin (I-19, Santa Cruz), anti-CHK1 (G-4, Santa Cruz), anti-CHK1 pS317 (Cell Signaling), anti-CHK2 pT68 (Y171, Epitomics), anti-CHK2 (EPR4325, Epitomics), anti-NBS1 pS343 (EP178, Epitomics), anti-NBS1 (A301-289A, Bethyl Laboratories), anti-ATR (N-19, Santa Cruz), anti-DNA-PK_cs (G-4, Santa Cruz), anti-KU80 (C-20, Santa Cruz), anti-KU70 (M-19, Santa Cruz), anti-DNA-PK_cs pS2056 (EPR5670, Epitomics), anti-CtIP (A300-488A, Bethyl Laboratories), and anti-GAPDH (0411, Santa Cruz).

Sowd G.A., Mody D., Eggold J., Cortez D., Friedman K.L, & Fanning E. (2014). SV40 Utilizes ATM Kinase Activity to Prevent Non-homologous End Joining of Broken Viral DNA Replication Products. PLoS Pathogens, 10(12), e1004536.

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Western Blot Analysis of DNA Repair Proteins

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Cell lysates were resolved by SDS-PAGE and transferred to a PVDF membrane. The following primary antibodies were used for immunoblot: Anti-Ku70 (C-19), anti Ku80 (C-20), and anti-NCL (MS-3) were from Santa Cruz Biotechnology (Dallas, TX); and anti YB-1 (ab76149) from Abcam. Primary antibodies were detected by incubation for 1 h with species-specific HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, Texas). Proteins were visualized using SuperSignal West Pico substrate (Pierce Biotechnology, Rockford, IL) and exposure to autoradiography film.

Li Y., Frederick K.M., Haverland N.A., Ciborowski P, & Belshan M. (2015). Investigation of the HIV-1 Matrix interactome during virus replication. Proteomics. Clinical applications, 10(2), 156-163.

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