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2 protocols using mouse anti gfp antibody

1

Molecular Characterization of DHAV-1 H Strain

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The DHAV-1 H strain (GenBank accession number: JQ301467.1), the engineered E. coli DH5α bacterium, HEK293T human embryonic kidney cells, and duck embryo fibroblasts (DEFs) used in this study were all preserved and provided by the Sichuan Agricultural University Poultry Disease Prevention Research Center. A mouse anti-Flag monoclonal antibody (Cat: M185-3S) and a mouse anti-HA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) and a mouse anti-GFP antibody (Cat: AE012) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), a HRP-conjugated goat anti-mouse IgG (Cat: A0216) and a HRP-conjugated goat anti-rabbit IgG (Cat: A0208) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358) and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory (Shen et al., 2016 (link)).
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2

Antibody Immunoblotting Protocol

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Mouse anti-puromycin antibody was obtained from Millipore. Rabbit anti-phospho-eIF2α (S51) and mouse anti-Myc antibody were obtained from Cell Signaling Technology. Mouse anti-HA and mouse anti-Flag antibody were purchased from MBL. Mouse anti-GFP antibody was obtained from ABclonal. Rabbit anti-beta (β)-actin antibody was obtained from Proteintech. HRP-conjugated goat anti-mouse IgG and HRP-conjugated goat anti-rabbit IgG were purchased from Beyotime. Rabbit anti-VP3 antibody was prepared in our laboratory (Shen et al., 2016 (link)).
C16 (PKR Inhibitor) and GSK2606414 (PERK inhibitor) were purchased from APExBIO, and GCN2-IN-1 (GCN2 inhibitor) was purchased from MCE. These inhibitors were dissolved in dimethyl sulfoxide (Sigma) and configured to 10 mmol/L, which were diluted to working concentration with MEM when used. Sodium arsenite was purchased from Sigma. Poly(I:C) was purchased from Invivogen.
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