Nod cg prkdc il2rg szj nsg mice

NOD.Cg-Prkdc Il2rg/SzJ (NSG) mice were purchased from The Jackson Laboratory. For JIMT-1^{39 (link)} cell line-derived xenografts, 6-week-old female mice were injected s.c in the flank with 5 × 10⁶ cells in 100 μl of matrigel. Two weeks later, when the tumors reached ~150 mm³, tumor-bearing mice were injected again with 5 × 10⁶ NK92 CARTIV+ cells i.t, i.v or s.c in matrigel in the neck. Two days later, tumors were dissociated for a single cell suspension using the gentleMACS Dissociator. For blood samples RBCs were lysed using ACK solution for 5 min at RT and washed using PBS. Samples were 70 µm strained, washed twice using PBS and suspended in PBS 2%FCS with DAPI 1 µg/mL and FACS measured using Beckman Coulter® Gallios™ flow cytometer. Data were analyzed using Kaluza™. Box plot was plotted using a python script utilizing the seaborn library. A two-tailed t test was performed using the scipy library. All animal experiments were carried out under the Institutional Animal Care and Use Committee (IACUC) of Ben-Gurion University of the Negev (BGU’s IACUC) according to specified protocols aimed to ensure animal welfare and to reduce suffering. The animal ethical clearance protocol numbers used for this study are IL-80-12-2015 and IL-29-05-2018.