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4 mercaptophenylboronic acid

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4-mercaptophenylboronic acid is a chemical compound that contains a phenyl ring with a boronic acid group and a sulfhydryl (thiol) group. It is commonly used as a reagent in organic synthesis and analytical chemistry applications.

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Lab products found in correlation

Silver nitrate, by Merck Group (2 mentions) D galactose, by Merck Group (2 mentions) D glucose, by Merck Group (2 mentions) Tryptone soya broth (tsb), by Thermo Fisher Scientific (1 mentions) 7 mercapto 4 methylcoumarin, by Merck Group (1 mentions) 1 dodecanthiol, by Merck Group (1 mentions) Tryptone soya agar tsa, by Thermo Fisher Scientific (1 mentions) Sodium borohydride, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Sulfuric acid, by Merck Group (1 mentions) Sodium citrate, by Merck Group (1 mentions) Nitric acid, by Merck Group (1 mentions) 4 mercaptobenzoic acid, by Merck Group (1 mentions) L glutathione reduced, by Merck Group (1 mentions) Bare fused silica capillary, by Molex (1 mentions) Phenylboronic acid, by Merck Group (1 mentions) Acs reagent, by Merck Group (1 mentions) D mannose, by Merck Group (1 mentions) D ribose, by Merck Group (1 mentions)

4 protocols using 4 mercaptophenylboronic acid

1

Synthesis and Characterization of Metallic Nanoparticles

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Gold(III) chloride trihydrate (~30 wt.% in HCl 99.99%), sodium borohydride (98%), L-ascorbic acid (AA) (≥ 99%), silver nitrate (99.8%), sodium citrate (≥ 99%), hydrochloric acid (≥ 37%), nitric acid (≥ 65%), sulfuric acid (95%), hydrogen peroxide (30 wt.%), ethanol (≥ 99.7%), toluen (≥ 99.7%)N-Dodecyl-N,N-dimethyl-3-ammoniun-1-propansulfunate (LSB; ≥ 99.7%), (3-aminopropyl)trimethoxysilane (APTES; ≥ 98.0%), L-glutathione, reduced (99%), 1-dodecanthiol (≥ 98%), 4-mercaptophenylboronic acid (≥ 99.7%), 4-mercaptobenzoic acid (99%) and 7-Mercapto-4-methylcoumarin (≥ 97%) were purchased from purchased from Sigma-Aldrich (St. Louis, MO, USA). Microscopy cover glass slides 21 mm × 6 mm were purchased from DEL Chimica (Milano, Italy).. All reagents were used as received. All the preparations were made with bidistilled water.
Tryptone Soya Broth (TSB) and Tryptone Soya Agar (TSA) for bacteria culture were purchased from Oxoid, Basingstoke, Hampshire, UK. S. aureus ATCC 6538 and Escherichia coli ATCC 10536 bacterial strains were used.
Rovati D., Albini B., Galinetto P., Grisoli P., Bassi B., Pallavicini P., Dacarro G, & Taglietti A. (2019). High Stability Thiol-Coated Gold Nanostars Monolayers with Photo-Thermal Antibacterial Activity and Wettability Control. Nanomaterials, 9(9), 1288.
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2

Carbohydrate and Boronate Affinity CE

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L-(+)-arabinose (≥ 99%), D-(−)-fructose, (≥ 99%), D-(+)-galactose (≥ 99%), D-(+)-glucose (≥ 99.5%, GC Graded), D-(+)-mannose (≥ 99%), D-(−)-ribose (≥ 99%), Phenylboronic acid (95%), 4-mercaptoPhenylboronic acid (90%), and sodium hydroxide pellets (≥ 97.0%, ACS Reagent) were purchased from Sigma Aldrich (St. Lous, MO). Ethanol (200 proof) was purchased from Decon Labs Inc. (King of Prussia, PA). Nanopure water (18.2 MΩ) was obtained from a Thermo Scientific Genpure system. Bare fused silica capillary with 75 μm inner diameter and 150 μm outer diameter was purchased from Polymicro Technologies (Pheonix, AZ).
Schorr H.C, & Schultz Z.D. (2023). Chemical Conjugation to Differentiate Monosaccharides by Raman and Surface Enhanced Raman Spectroscopy. The Analyst, 148(9), 2035-2044.
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3

Preparation and Characterization of Glycolipid Nanostructures

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Silver nitrate (AgNO3, ≥99%), anhydrous 1,5-pentanediol (PD, ≥97%), poly(vinylpyrrolidone) (PVP, average Mw = 55,000 g mol−1), 1H,1H,2H,2H-perfluorodecanethiol (PFDT, ≥97%), 4-mercaptophenylboronic acid (4-MPBA, ≥90%) and, dodecane (C12H26, anhydrous, ≥99%), D-(+)-galactose (C6H12O6, ≥99%), D-(+)-glucose (dextrose C6H12O6, ≥99.5%, GC) were purchased from Sigma Aldrich. Copper (II) chloride was purchased from Alfa Aesar. Glucosylceramides (GlcCer C8), GlcCer(β) ceramide (d18:1/8:0 ≥ 99%); GlcCer C12, GlcCer(β) ceramide (d18:1/12:0 ≥ 99%); GlcCer C16, GlcCer(β) ceramide (d18:1/16:0 ≥ 99%); GlcCer C18, GlcCer(β) ceramide (d18:1/18:0 ≥ 99%); GlcCer C24:1 GlcCer(ß) ceramide (d18:1/24:1(15Z) ≥ 99%) and galactosylceramide (GalCer C8), GalCer(β) ceramide (d18:1/8:0 ≥ 99%); GalCer C12 GalCer(β) ceramide (d18:1/12:0 ≥ 99%); GalCer C16 GalCer(β) ceramide (d18:1/16:0 ≥ 99%); GalCer C18 GalCer(β) ceramide (d18:1/18:0 ≥ 99%); GalCer C24 GalCer(β) ceramide (d18:1/24:0 ≥ 99%); GalCer C24:1 GalCer(ß) ceramide (d18:1/24:1(15Z) ≥ 99%) were purchased from Avanti® Polar Lipids, Inc. Ethanol (C2H6O, ACS, ISO, Reag. Ph Eur), acetone (C3H6O, HPLC, ≥99.9%) and potassium hydroxide (KOH, ACS reagent, ≥85%, pellets) were obtained from Merck. Milli-Q water (>18.0 MΩ cm) was purified with a Sartorius Arium® 611 UV ultrapure water system. All reagents were used without further purification.
Tan E.X., Leong S.X., Liew W.A., Phang I.Y., Ng J.Y., Tan N.S., Lee Y.H, & Ling X.Y. (2024). Forward-predictive SERS-based chemical taxonomy for untargeted structural elucidation of epimeric cerebrosides. Nature Communications, 15, 2582.
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4

Proteasome Subunit Assay Protocol

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The reagents were of analytical grade and were used without further purification. Millipore Milli-Q nanopure water (resistivity ≥ 18 MΩ cm) was used for the preparation of all solutions.
Proteasome 20S (human) and 20S β5 subunit (human) monoclonal antibody (Abβ), 20S core subunits polyclonal antibody (Abcore) and 20S substrate carbobenzoxy(Z)-Leu(L)-Leu(L)-Glu(E)-7-amino-4-methylcoumarin (AMC) of caspase activity were from Enzo Life Sciences. The 4-mercaptophenylboronic acid, AmPhP, AmPh, Tris/HCl, KCl, NaCl, MgCl2, SDS, EDC, NHS, PBS with 0.1% Tween, and Fetal Bovine Serum with ≤10 EU/mL endotoxins were from Sigma-Aldrich. Alkaline Phosphatase Labeling Kit-NH2 was from Abnova (St. Louis, MO, USA). The labeling of Abcore with AlkP was done in agreement with the instructions in the labeling kit manual.
Stock solutions of Z-LLE-AMC, AmPh and AmPhP were prepared in dimethyl sulfoxide (DMSO). Stock solutions of 20S proteasome were prepared in water and kept at −20 °C until further utilization.
The proteasome assay buffer (PAB) pH = 7.5 contained 50 mM Tris/HCl, 25 mM KCl, 10 mM NaCl, 1 mM MgCl2 and 100 µM SDS.
Barsan M.M., Sanz C.G., Onea M, & Diculescu V.C. (2021). Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome. Sensors (Basel, Switzerland), 21(8), 2702.
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